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安全信息

AMPD1

Sigma-Aldrich

脱氧核糖核酸酶I

Amplification Grade

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别名:
脱氧核糖核酸酶 I（DNA酶I）
EC 号:
3.1.21.1 (BRENDA, IUBMB)
NACRES:
NA.55

形式

liquid

浓度

1 unit/μL

颜色

colorless

运输

wet ice

储存温度

−20°C

相关类别

一般描述

脱氧核糖核酸酶I（DNase I）是一种从牛胰腺中分离的内切核酸酶，可将双链和单链DNA消化成寡核苷酸和单核苷酸。 扩增级 DNase I 已纯化去除RNase活性，适于在 RT-PCR（逆转录酶 - 聚合酶链反应）等敏感应用之前去除 RNA 制剂中的DNA。

DNase I可将双链和单链DNA酶解成寡核苷酸和单核苷酸。采用提供的反应缓冲液，在室温下消化 15 分钟，可去除 RNA 制剂中的 DNA。然后随同终止液加热，使DNase I失活。加热还会使RNA中的发夹变性，可直接用于逆转录。

当前没有任何 RNA 分离程序可以 100% 去除 DNA。 许多商业DNase I 制剂受到了残留RNase污染。 这种RNase污染会在逆转录以前破坏或分解有价值的RNA样品。实验室比较表明，Sigma′的扩增级 DNase I 的RNase活性低于几家领先的分子生物学产品供应商产品。

应用

扩增级脱氧核糖核酸酶I（DNase I）已用于：
  • 在 RNA 的分离和纯化过程中消化 DNA。纯化的 RNA 可用于通过RNA 逆转录酶合成 cDNA。
  • 水解细胞外基质 (ECM) 成分并增强光敏剂在生物膜中的渗透以确定抗菌光动力疗法 (aPDT) 对白色念珠菌生物膜的功效
  • 从牛血样中提取的总 RNA 中去除污染 DNA

特点和优势

  • 适用于去除 RNA 中的 DNA
  • 超低RNase活性
  • 经过优化的10×反应缓冲液和终止液，可使DNase I完全失活

适用性

适用于去除 RNA 制剂中的 DNA。

单位定义

在 37 °C 下，一个单位可在 10 min 内将 1μg 质粒DNA 消化为寡核苷酸。

法律信息

购买本产品附带有限的许可，仅可用于研究用途聚合酶链式反应 (PCR) 过程，且应配合热循环仪使用。该热循环仪在 PCR 过程的自动执行中的使用由预先许可费涵盖，该费用通过向 Applied Biosystems 支付或购买的方式（即授权的热循环仪）支付。

抑制剂

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价格

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储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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示例

T1503
货号
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25G
包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

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  • 如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。

  • 如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。

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部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。

索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the concentration of the DNAse in Product AMPD1, DNase I?

    The DNAse is provided at 1 unit/uL in 1 mL total volume.

  4. What do I do if my DNA is not completely digested after 15 minute digestion at room temperature when using Product AMPD1, DNase I?

    If further digestion is required, incubation can be performed for 30 minutes at 37°C.

  5. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Leonie Doorduin et al.
DNA research : an international journal for rapid publication of reports on genes and genomes, 18(2), 93-105 (2011-03-30)
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Hafid Ait-Oufella et al.
The Journal of experimental medicine, 207(8), 1579-1587 (2010-07-07)
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John P Dunbar et al.
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The noble false widow spider Steatoda nobilis originates from the Macaronesian archipelago and has expanded its range globally. Outside of its natural range, it may have a negative impact on native wildlife, and in temperate regions it lives in synanthropic
Ling Yuan et al.
Molecular vision, 18, 1684-1695 (2012-07-10)
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