产品名称
BME培养基, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: no
NaHCO3: yes
Earle’s salts (5% CO2): yes
shipped in
ambient
storage temp.
2-8°C
General description
Eagle基础培养基(BME)是应用最广泛的合成细胞培养基之一。组织培养协会建议,术语“Eagle基础培养基”仅用于指代专为支持海拉细胞而开发的配方。BME先前已用于单层培养研究,以评估正常(WI-38)和转化(小鼠和海拉)细胞的生长反应。BME是杜氏改良Eagle培养基(DME)和Eagle最低必需培养基(MEM)的前体。
Application
Eagle培养基已用于:
- 作为耳蜗器官型培养物和水杨酸治疗的培养基组分
- 维持小脑颗粒神经元(CGN),以确定是否存在调节轴突线粒体分布和/或群体动态的系统
- 制备用于切片叠加分析的皮层切片、无血清培养基和切片培养基,以研究细胞外信号对神经元发育的影响
Preparation Note
添加 0.292g/L L-谷氨酰胺。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
钙是一种离子稳定的二价阳离子,在细胞培养基中具有有益和有毒的双重属性,可作为一系列细胞培养基的组成部分。
用于传代和分装悬浮细胞系的细胞培养方案
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.