grade
Molecular Biology
Quality Level
description
Mode of use Voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. User should test their chamber to determine optimum voltage.
form
solution
concentration
10 ×
impurities
DNase, RNase and nickase, none detected
pH
8.0-8.5 (20-25 °C)
storage temp.
room temp
General description
Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.
Application
Suitable for:
- Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
- Use with TBA and TAE gels
- Preparation of agarose gels
Features and Benefits
- Faster run times
- Sharper bands
- Easy to dilute and use
- Flexible use with a variety of gel types
Packaging
The 4 L and 10 L sizes are supplied in dispensers with a spigot.
Legal Information
Bionic is a trademark of Sigma-Aldrich Co. LLC
Disclaimer
Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B
存储类别
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
实验方案
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.