Human Cortical Glutamatergic Neurons

Human iPSC-Derived Cortical Glutamatergic Neurons. GFP-expressing neurons also available (BX-0301-30-1M). Required supplements for the final cell differentiation and maturation (sold separately): BX-2300-100uL, BX-2020-100uL, BX-2040-100uL. Glutamatergic neurons product glutamate, one of the main excitatory neurotransmitters in the central nervous system (CNS). These neurons are involved with learning and memory functions and are used to develop therapeutics for diseases such as Alzheimer’s disease, Huntington’s disease, epilepsy, Parkinson’s disease, and more.

Function: Cortical Glutamatergic Neurons exhibit pronounced electrophysiological activity after two weeks in culture, as demonstrated by multi-electrode array (MEA) recordings.

Marker Expression: Fully differentiated Cortical Glutamatergic Neurons have high neuronal purity (>90%) and comprise predominantly excitatory neurons. Labeling with the pan-neuronal marker MAP2 (red) highlights the neuronal purity of these cells. Labelling with the glutamatergic-specific markers CTIP2 (green) and TBR1 (blue) indicates overwhelming presence of glutamatergic neurons. Morphology: Cortical Glutamatergic Neurons exhibit substantial neurite outgrowth within a week in culture and are adherent. Calcein staining (green) demonstrates the characteristic oval cell shape and long processes of Cortical Glutamatergic Neurons in culture. Primary neurotransmitter released: Glutamate.