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安全信息

C4129

Sigma-Aldrich

α-糜蛋白酶 来源于牛胰腺

Type II, lyophilized powder, ≥40 units/mg protein

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别名:
α-chymotrypsin A and B, alpha-chymotrypsin
CAS号:
9004-07-3
EC 号:
3.4.21.1 (BRENDA, IUBMB)
EC 号:
232-671-2
MDL编号:
MFCD00130481
eCl@ss:
42010112

类型

Type II

形式

lyophilized powder

比活

≥40 units/mg protein

分子量

25 kDa

组成

protein, ≥85%

颜色

white to off-white

UniProt登记号

P00767

应用

diagnostic assay manufacturing

储存温度

−20°C

基因信息

cow ... CTRB1(618826)

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相关类别

应用

sigma 公司的酶已被用于研究糖基化 α-胰凝乳蛋白酶的结构 - 功能关系，使用固定化金属离子亲和层析（IMAC）和固定化金属离子亲和毛细管电泳（IMACE）。

生化/生理作用

α-胰凝乳蛋白酶是丝氨酸肽酶，并且具有通过二硫键连接的三条多肽链（A 链-13 残基，B 链-131 残基和 C 链-97 残基）中包含的 241 个氨基酸残基。发现该酶的分子量为 25kDa。pI 是 8.75。它选择性地水解酪氨酸、苯丙氨酸、色氨酸和亮氨酸的 C-末端侧的肽键。Ca2+ 激活和稳定酶。该酶被二异丙基氟磷酸盐（DFP）、甲苯磺酰氟（PMSF）、N-对甲苯磺酰基-L-苯丙氨酸氯甲基酮（TPCK）、胰凝乳蛋白酶抑制剂、抑肽酶、α1-抗胰蛋白酶和 α2-巨球蛋白、10mM Cu2+ 和 Hg2+ 抑制。
一种丝氨酸蛋白酶，能将与芳香烃或其他大型疏水性侧链（Tyr、Trp、Phe、Met、Leu）结合的肽从键的羧基端水解。

单位定义

一个单元将在 pH 7.8，25℃下，每分钟水解 1.0 μmole BTEE。

制备说明

以 3×结晶的胰凝乳蛋白酶原为原料生产

分析说明

通过 E1％/280 确定的蛋白质

其他说明

有关胰凝乳蛋白酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer

应用

产品编号
说明
价格

底物

产品编号
说明
价格

抑制剂

产品编号
说明
价格

A2504

6-氨基己酸, ≥99% (titration), powder

A7824

6-氨基己酸, BioUltra, ≥99%

D0879

氟磷酸二异丙酯

L2023

亮肽素 三氟乙酸盐, ≥90% (HPLC), microbial

T9777

胰蛋白酶-胰凝乳蛋白酶抑制剂 来源于大豆, lyophilized powder

A1153

抑肽酶 来源于牛肺, lyophilized powder, 3-8 TIU/mg solid

P7626

苯甲磺酰氟, ≥98.5% (GC)

B1552

溴烯醇内酯, ≥98% (TLC)

A9024

α1-抗胰蛋白酶 来源于人类血浆, salt-free, lyophilized powder

A6150

α1-抗胰蛋白酶 来源于人类血浆, salt-free, lyophilized powder

A9285

α1-Antichymotrypsin 来源于人类血浆, ≥92.5% (SDS-PAGE), lyophilized powder

T4376

N-对-甲苯磺酰基-L-苯基乙基氯甲基酮, ≥97% (TLC), powder

C7268

胰凝乳蛋白酶抑制剂, microbial

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 1

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

常规特殊物品

分析证书(COA)

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示例

T1503
货号
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25G
包装规格/数量

其它示例：

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MMYOMAG-74K-13

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the molecular weight of alpha-chymotrypsin from bovine pancreas, product C4129?

    The molecular weight of alpha chymotrypsin, reported in the literature, is approximately 25 kDa.

  6. How do you recommend preparing a solution of Product No. C4129?

    It is recommended to reconstitute Product No. C4129 in 1 mM hydrochloric acid containing 2 mM calcium chloride. The calcium functions as a stabilizer, and possibly an activator, of the enzyme.

  7. How can solutions of alpha chymotrypsin (Product No. C4129) be stored?

    Stock solutions prepared in 1 mM HCl, containing 2 mM calcium chloride, can be stored at -20 ° for about one week.

  8. What is the pH optimum for the enzyme, Product No. C4129?

    The pH optimum for alpha-chymotrypsin is between pH 7.5-8.5. Please see the following reference: Wilcox, P.E., Chymotrypsingogens-chymotrypsins, Methods in Enzymology, 19, 64-80 (1970).

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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To investigate the change of bioavailability and structure of myofibrillar proteins during Nanjing dry-cured duck processing, carbonyl content, sulfhydryl (SH) group, disulfide (SS) group, sodium dodecyl sulfate polyacrylamide gel electrophoresis, surface hydrophobicity, secondary structures and in vitro digestibility were determined.
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Epstein-Barr virus (EBV) is a causative agent of infectious mononucleosis and is associated with 200,000 new cases of cancer and 140,000 deaths annually. Subunit vaccines against this pathogen have focused on the gp350 glycoprotein and remain unsuccessful. We isolated human
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Electrospray ionization (ESI) ion trap mass spectrometers with relatively low resolution are frequently used for the analysis of natural products and peptides. Although ESI spectra of multiply charged protein molecules also can be measured on this type of devices, only
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International journal of molecular sciences, 20(11) (2019-06-05)
The serine protease Caseinolytic protease subunit P (ClpP) plays an important role for protein homeostasis in bacteria and contributes to various developmental processes, as well as virulence. Therefore, ClpP is considered as a potential drug target in Gram-positive and Gram-negative
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商品

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

Chymotrypsin

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

实验方案

Procedure for Enzymatic Assay of α-Chymotrypsin (EC 3.4.21.1)

Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

Chromatograms

application for HPLC

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