Merck
CN
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文件

C7902

Sigma-Aldrich

氯化钙 二水合物

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

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经验公式（希尔记法）:
CaCl2 · 2H2O
CAS号:
10035-04-8
分子量:
147.01
EC 号:
233-140-8
MDL编号:
MFCD00149613
PubChem化学物质编号:
24892994
NACRES:
NA.71

产品线

BioReagent

检测方案

≥99.0%

形式

powder

技术

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

mp

176 °C (dec.) (lit.)

溶解性

H2O: 294 mg/mL, clear, colorless

SMILES字符串

O.O.Cl[Ca]Cl

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2

InChI key

LLSDKQJKOVVTOJ-UHFFFAOYSA-L

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相关类别

一般描述

氯化钙（CaCl2）是一种常用的生化试剂。它在信号转导、肌肉收缩、维持细胞膜和细胞壁稳定等生物学过程中发挥着重要作用。CaCl2 用于大肠杆菌（E. Coli）的制备和转化，以及真核细胞的质粒）的制备和转化，以及真核细胞与质粒 DNA 或高分子量基因组 DNA 的转染。已有研究显示质粒 DNA pBR322 可通过 CaCl2 介导的电穿孔法导入大肠杆菌。CaCl2 用于大肠杆菌 NADH-泛醌氧化还原酶（络合物 I）的稳定和二维结晶。在 CaCl2 和柠檬酸钠环境下，猪胰腺弹性蛋白酶的结晶揭示了蛋白质金属结合位点的钙结合。CaCl2 对大肠杆菌微管蛋白样丝状温度敏感突变体 Z（FtsZ）分裂蛋白聚合过程中总荧光强度的影响已通过多孔荧光检测法进行了研究。

应用

二水合氯化钙已用于：
  • 培养基制剂
  • 精子收集和制备
  • 细胞培养和原生质体制备

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H319

危险分类

Eye Irrit. 2

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

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Hemolymph analysis and evaluation of newly formulated media for culture of shrimp cells (Penaeus stylirostris)
Shimizu C, et al.
In Vitro Cellular & Developmental Biology. Animal, 37(6), 322-329 (2001)
The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and embryonic development (IVC) of sheep oocytes
PANCARCI S M, et al.
Kafkas U?niversitesi veteriner faku?ltesi dergisi, 16(5) (2010)
Marco Wallenborn et al.
PloS one, 15(7), e0234986-e0234986 (2020-07-08)
Glioblastoma is a common, malignant brain tumor whose disease incidence increases with age. Glioblastoma stem-like cells (GSCs) are thought to contribute to cancer therapy resistance and to be responsible for tumor initiation, maintenance, and recurrence. This study utilizes both SNP
Zhuqing Wang et al.
Mobile DNA, 10, 17-17 (2019-05-11)
Transposable elements (TEs) make up > 50% of the human genome, and the majority of retrotransposon insertions are truncated and many are located in introns. However, the effects of retrotransposition on the host genes remain incompletely known. We report here that
Biomolecule delivery into canola protoplasts by centrifuging cells with microbubbles
He C, et al.
Febs Letters, 587(3), 285-290 (2013)
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