Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
Dulbecco′s改良Eagle′s培养基/Ham F-12营养混合物是1:1混合物。
Application
Dulbecco′s改良Eagle′s培养基/Ham F-12营养混合物已用于:
- 小鼠胚胎脑皮质培养
- 作为人角膜上皮(HCE)培养基组分,培养人角膜缘外植体
- 人角膜上皮细胞系培养
Preparation Note
按照每升培养基12.0克粉末配制。
补充 1.2 g/L 碳酸氢钠
Other Notes
微量元素包括钼酸铵、偏钒酸铵、氯化亚锡、亚硒酸钠、硫酸镁、氯化镍和偏硅酸钠
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实验方案
粉末培养基及盐混合物极具吸湿性,应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
商品
Nutrient Mixture F-12 Ham Formulation