D2532
Denhardt溶液 50x
for Northern and Southern blotting, solution
别名:
blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution
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关于此项目
NACRES:
NA.25
UNSPSC Code:
12161700
Quality Level
form
solution
technique(s)
Southern blotting: suitable
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
−20°C
General description
Denhardt溶液是一种封闭剂，常规用于减少检测试剂与Northern和Southern印迹膜的非特异性结合。 这种50x浓缩液可以稀释到任何浓度，用于多种预杂交和杂交缓冲液。
Preparation Note
Denhardt溶液是1%牛血清白蛋白（BSA）、1%Ficoll和1%聚乙烯吡咯烷酮（PVP）的水溶液。
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
此项目有
实验方案
Ficoll 400®是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。Ficoll 400®是完全非离子性的。由于其含有丰富的羟基，Ficoll 400®具有很强的亲水性和水溶性。
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.