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安全信息

D5671

Sigma-Aldrich

培养基

With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

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别名:
DME, DMEM
NACRES:
NA.75

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

L-glutamine: no
glucose: high
NaHCO3: yes
sodium pyruvate: no
phenol red: yes
HEPES: no

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

这种DMEM高葡萄糖培养基是一种不含添加因子（常见修改）如L-丙氨酰-L-谷氨酰胺、HEPES或丙酮酸钠的1x完全培养基。 它与原始DMEM高葡萄糖配方的不同点在于用吡哆醇代替了吡哆醛。 吡哆醛是一种不稳定的培养基成分。 该培养基需要添加L-谷氨酰胺或于L-丙氨酰-L-谷氨酰胺。

应用

Dulbecco′s Modified Eagle′s Medium（DMEM）是对Basal Medium Eagle (BME)的一种改良，含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此，它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养，以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外，葡萄糖浓度也被提高至4500 mg/L，因此被命名为“DMEM/High”。

重悬

添加0.584 g/L L-谷氨酰胺。

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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