recombinant
expressed in E. coli
Quality Level
conjugate
(N-linked)
form
solution
specific activity
30 U/mg
mol wt
32 kDa
shipped in
wet ice
storage temp.
2-8°C
Application
来自 和平空间站伊金氏菌 的内切糖苷酶 F3 已用于分析血清蛋白的核心岩藻糖基化和胰蛋白酶消化物。
Biochem/physiol Actions
内切糖苷酶 F3 属于糖苷水解酶家族 18 (GH18)。具有水解活性。内切糖苷酶 F3 糖基化 α-1,6-岩藻糖基化 GlcNAc 衍生物,得到天然核心岩藻糖基化复合物型 N -糖肽。
裂解天冬酰胺连接的双天线和三天线复合物,寡糖取决于核心岩藻糖基化和肽键的状态。
Packaging
提供 5× 反应缓冲液,250 mM 醋酸钠,pH 4.5
Physical form
20 mM Tris-HCl,pH 7.5中的无菌填充溶液
Other Notes
一个单元将在37°C pH 4.5 下每分钟从 1μmole 变性猪纤维蛋白原释放 N-连接寡糖。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
商品
Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.
Characterization of novel endo-beta-N-acetylglucosaminidases from Sphingobacterium species, Beauveria bassiana and Cordyceps militaris that specifically hydrolyze fucose-containing oligosaccharides and human IgG
Huang Y, et al.
Scientific reports, 8(1), 246-246 (2018)
Chemical Biology of Glycoproteins (2017)
Advances in Carbohydrate Chemistry (2016)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| E2264-.2UN | 04061833602560 |