Merck
CN

E3004

Sigma-Aldrich

提取-N-Amp PCR ReadyMix

Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

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别名:
Plant direct PCR master mix, Tissue direct PCR master mix
NACRES:
NA.55

用途

sufficient for 100 reactions
1.2 mL sufficient for 100 amplifications
sufficient for 1000 reactions
12 mL sufficient for 1000 amplifications
sufficient for 10000 reactions
125 mL sufficient for 10000 amplifications

特点

dNTPs included
hotstart

颜色

colorless

application(s)

agriculture

运输

wet ice

储存温度

−20°C

一般描述

Extract-N-Amp PCR ReadyMix是一种专门配制的热启动PCR预混液,可直接以提取物进行PCR扩增。
PCR ReadyMix 预期与 Sigma 的 Extract-N-Amp Plant PCR 试剂盒和 Extract-N-Amp Tissue PCR 试剂盒配套使用。 所有 Extract-N-Amp 试剂盒均包括 PCR ReadyMix,每次提取可充分进行一次 PCR 反应。但是,如果需要额外的 PCR 反应,可能需要补充 PCR ReadyMix。

应用

Extract-N-Amp PCR ReadyMix已被用于以下用途:
  • 基因组 DNA 提取
  • 真菌 DNA提取和聚合酶链反应(PCR)扩增
  • 直接 PCR 扩增
  • 基因分型
  • 实时PCR

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护:5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品,即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可,即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利,包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途,需另外征得Roche许可。有关购买许可的更多信息,可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

相关产品

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Muhammad Iqbal et al.
Genome, 50(5), 511-516 (2007-07-07)
Vernalization response (Vrn) genes play a major role in determining the flowering/maturity times of spring-sown wheat. We characterized a representative set of 40 western Canadian adapted spring wheat cultivars/lines for 3 Vrn loci. The 40 genotypes were screened, along with
Melinda Belisle et al.
Microbial ecology, 63(4), 711-718 (2011-11-15)
Microfungi that inhabit floral nectar offer unique opportunities for the study of microbial distribution and the role that dispersal limitation may play in generating distribution patterns. Flowers are well-replicated habitat islands, among which the microbes disperse via pollinators. This metapopulation
Huirong Gao et al.
Frontiers in plant science, 11, 535-535 (2020-05-21)
Modern maize hybrids often contain biotech and native traits. To-date all biotech traits have been randomly inserted in the genome. Consequently, developing hybrids with multiple traits is expensive, time-consuming, and complex. Here we report using CRISPR-Cas9 to generate a complex
Anneke van der Zee et al.
BMC research notes, 4, 150-150 (2011-05-28)
To facilitate automation, a novel DNA extraction method for MRSA was adopted. The MRSA specific chromosome-SCCmec PCR was adapted, additional primers were added, and the performance was validated. From various laboratories in The Netherlands we received a total of 86
Beika Zhu et al.
Marine drugs, 17(2) (2019-01-27)
Cell cycle reentry is a unified mechanism shared by several neurodegenerative diseases, including Alzheimer's disease (AD) and Ataxia Telangiectasia (A-T). This phenotype is often related to neuroinflammation in the central nervous system. To mimic brain inflammation in vitro, we adopted

商品

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit

The Extract-N-Amp™ Tissue PCR Kit has been created to release PCR-ready DNA from mouse tails in a 15 minute single tube procedure. The included 2x PCR mix is optimized to work with the crude extracts and the solutions in the kit

Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.

Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.

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