产品名称
营养混合物 F-12 Ham, Coon′s Modification, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
components
phenol red: 0.00125 g/L
L-glutamine: 0.292 g/L
sodium pyruvate: 0.22 g/L
glucose: 1.802 g/L
shipped in
ambient
storage temp.
2-8°C
Application
库恩对哈姆氏 F-12 的改造是为培养通过病毒融合产生的杂交细胞而开发的。修饰包括使氨基酸和丙酮酸加倍，并包括抗坏血酸。盐浓度也发生了改变。配方中含有 0.863 mg/L 硫酸锌，这可能使其不适合培养小鼠 L -细胞。
Preparation Note
按照每升培养基11.5克粉末配制。
补充2.68 g/L碳酸氢钠。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.