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F9291

Sigma-Aldrich

抗FLAG® BioM2单克隆抗体 小鼠抗

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

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别名:
单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk

生物来源

mouse

偶联物

biotin conjugate

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

M2, monoclonal

形式

buffered aqueous glycerol solution

种属反应性

all

浓度

~1 mg/mL

技术

dot blot: suitable (chemiluminescent detection)

同位素/亚型

IgG1

免疫原序列

DYKDDDDK

运输

dry ice

储存温度

−20°C

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相关类别

一般描述

FLAG BioM2小鼠单克隆抗体通过酰肼键与生物素共价连接。该抗体可识别FLAG融合蛋白N末端、Met-N末端或C末端的FLAG序列。

应用

生物素标记抗体被用于使用亲和素或链霉亲和素偶联报告酶（如链霉亲和素-过氧化物酶）的免疫检测方法。 当使用小鼠细胞作为重组蛋白宿主时，首选一抗偶联物。
抗体适用于免疫荧光、蛋白质免疫印迹、显微镜应用和亲和素-生物素复合物的形成。

请访问我们的 FLAG® 文献 门户，浏览其他应用参考文献。

外形

溶于50％甘油，10 mM磷酸钠，pH 7.25，含有150mM NaCl和0.02％叠氮化钠

制备说明

用TBS（0.05M Tris，pH7.4，含有0.15M NaCl）稀释抗体至终浓度为1-10μg/mL。

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Hong-Won Lee et al.
Nature communications, 4, 1505-1505 (2013-02-21)
Co-immunoprecipitation (co-IP) has become a standard technique, but its protein-band output provides only static, qualitative information about protein-protein interactions. Here we demonstrate a real-time single-molecule co-IP technique that generates real-time videos of individual protein-protein interactions as they occur in unpurified
Yoori Kim et al.
Scientific reports, 7(1), 2071-2071 (2017-05-20)
Single-molecule studies of protein-nucleic acid interactions frequently require site-specific modification of long DNA substrates. The bacteriophage λ is a convenient source of high quality long (48.5 kb) DNA. However, introducing specific sequences, tertiary structures, and chemical modifications into λ-DNA remains technically
Helen Hwang et al.
Scientific reports, 4, 6391-6391 (2014-09-30)
The ends of eukaryotic chromosomes are capped by telomeres which consist of tandem G-rich DNA repeats stabilized by the shelterin protein complex. Telomeres shorten progressively in most normal cells due to the end replication problem. In more than 85% of
Qing Xu et al.
Nature communications, 8(1), 425-425 (2017-09-06)
Tumor necrosis factor (TNF) has a critical role in diverse cellular events including inflammation, apoptosis and necroptosis through different signaling complexes. However, little is known about how the transition from inflammatory signaling to the engagement of death pathways is modulated.
Kenichiro Kawai et al.
PloS one, 6(11), e27106-e27106 (2011-11-11)
Nanoparticles (NPs) are small entities that consist of a hydroxyapatite core, which can bind ions, proteins, and other organic molecules from the surrounding environment. These small conglomerations can influence environmental calcium levels and have the potential to modulate calcium homeostasis
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Glycan Labeling

Structural modifications of proteins are essential to living cells. When aberrantly regulated they are often the basis of disease. Glycans are responsible for much of the structural variation in biologic systems, and their representation on cell surfaces is commonly called the “glycome.”

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