type
Type H-1
Quality Level
form
partially purified powder
specific activity
≥300,000 units/g solid
secondary activity
≥10,000 units/g solid sulfatase
storage temp.
−20°C
General description
β-葡萄糖醛酸酶(罗马蜗牛来源H-1型)是来源于罗马蜗牛的部分纯化粉末。来自软体动物的多种β-葡糖醛酸糖苷酶也含有硫酸酯酶活性。
Application
Biochem/physiol Actions
在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解
Analysis Note
来自软体动物的多种β-葡糖醛酸糖苷酶也含有硫酸酯酶活性。
Other Notes
在pH 5.0、温度37°C条件下,一单元sigma或改良的Fishman单元1小时会从酚酞葡糖苷酸释放 1.0 μg酚酞(30分钟实验)。
硫酸酯酶单元定义:一单位的硫酸酯酶每小时,pH5.0,37℃能够水解1.0 μmole p-硝基儿茶酚硫酸盐。
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signalword
Danger
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk
WGK 1
法规信息
低风险生物材料
此项目有
实验方案
为了使用β-葡糖醛酸酶优化水解,必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估,例如孵育时间、温度、水解pH、酶源和酶浓度。
酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)适用于可检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶的所有产品。应由经过培训的分析服务实验室人员负责按照书面规定执行此程序。
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
商品
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G0751-500KU | 04061835556953 |
| G0751-100KU | 04061833624142 |
| G0751-1MU | 04061833624159 |
| G0751-2MU | 04061833624166 |
