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G1279

G 418 二硫酸盐

powder, suitable for plant cell culture, BioReagent

别名:

抗生素G418

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关于此项目

经验公式（希尔记法）:
C20H40N4O10 · 2H2SO4
化学文摘社编号:
108321-42-2
分子量:
692.71
MDL number:
MFCD00058314
UNSPSC Code:
10171502
PubChem Substance ID:
24895058
NACRES:
NA.72
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产品名称

G 418 二硫酸盐, suitable for plant cell culture, BioReagent

product line

BioReagent

Quality Level

200

form

powder

potency

≥720 μg per mg (Dried basis)

technique(s)

cell culture | plant: suitable

color

white to off-white

solubility

100 mg/mL (H2O clear)

antibiotic activity spectrum

Gram-negative bacteria

application(s)

agriculture

mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

General description

Chemical structure: aminoglycoside

Application

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Preparation Note

G418 powder is soluble in water at 100 mg/mL.

Disclaimer

The product is stable for three years when stored at 2-8°C.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

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实验方案

Antibiotics in Plant Tissue Culture Protocol

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

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Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic

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