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安全信息

G1N10

Sigma-Aldrich

GenElute™ 哺乳动物基因组DNA小量制备试剂盒

sufficient for 10 purifications

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别名:
哺乳动物基因组DNA小量制备, Gen Elute
NACRES:
NA.55

用途

sufficient for 10 purifications

储存温度

15-25°C

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相关类别

一般描述

GenElute哺乳动物基因组DNA纯化试剂盒可以简单、方便地从多种哺乳动物源样本中分离高纯度的大分子量DNA。此类试剂盒使用了为基因组DNA纯化精选的硅胶膜，采用了方便的离心柱形式。

可从培养细胞、组织（包括啮齿动物尾巴）以及新鲜的全血或白血细胞之中分离高纯度的基因组DNA。该试剂盒将硅胶膜技术的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。

应用

纯化所得的基因组DNA可直接供以下下游应用使用：
  • 限制性内切酶酶切
  • PCR
  • Southern印迹
  • 测序反应
  • 克隆
  • 连接

特点和优势

  • 预期得率：25 μg / 2 x 106个培养细胞；30 μg / 25 mg组织
  • 洗脱体积：200 - 400 μl
  • 所需时间：细胞裂解后20分钟
  • A260/A280比率：1.6 - 1.9
  • 是否需要机械式均质化：否

原理

起始材料首先使用含有离液盐的溶液进行裂解，以确保大分子彻底变性。之后加入乙醇，裂解物离心通过微量离心管时，DNA会结合到硅胶模上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。预期的基因组DNA得率很大程度上取决于所用的起始材料用量及类型。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。

其他说明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
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  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology化学品安全说明书
  • R6148RNase A solution化学品安全说明书

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警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2

储存分类代码

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

闪点(°F)

483.8 °F

闪点(°C)

251 °C

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