Merck
CN

G7396

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于大肠杆菌

Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)

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别名:
β-D-葡萄糖苷酸葡萄糖醛酸水解酶
CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

类型

Type IX-A

质量水平

形式

lyophilized powder

比活

1,000,000-5,000,000 units/g protein (30 min assay)

分子量

290 kDa

组成

Protein, 30-70% biuret

运输

wet ice

储存温度

−20°C

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应用

比较不同酶性能的新技术文章
了解更多
了解Sigma研发获得的最新应用数据,关于使用不同来源的酶优化不同药物类别的水解,并使用色谱纯化酶降低酯酶活性导致6-单乙酰吗啡(6-MAM)转化为吗啡的影响
有效水解类固醇葡糖苷酸。

质量

含有缓冲盐和稳定剂。

单位定义

一个Sigma或改良Fishman单位是指在pH 6.8、温度37°C条件下(30分钟测定),每小时从苯酚葡萄糖醛酸中释放出1.0 μg酚酞所需的酶量。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

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示例

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商品

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Today, diverse studies report the benefits of probiotics, such as inhibitory effects on pathogens, aid in the management or prevention of chronic intestinal inflammatory diseases or atopic syndromes, and support to the immune system. Potential beneficial applications abound, researchers continue to evaluate the effictiveness and clarify the mechanisms of action of probiotics.

Today, diverse studies report the benefits of probiotics, such as inhibitory effects on pathogens, aid in the management or prevention of chronic intestinal inflammatory diseases or atopic syndromes, and support to the immune system. Potential beneficial applications abound, researchers continue to evaluate the effictiveness and clarify the mechanisms of action of probiotics.

实验方案

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

To optimize hydrolysis using β-glucuronidase, factors such as incubation time, temperature, hydrolysis pH, enzyme source, and enzyme concentration must be evaluated for each glucuronide metabolite to be analyzed.

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