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安全信息

G8295

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于大肠杆菌

≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder

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别名:
β-D-葡萄糖苷酸葡萄糖醛酸水解酶
CAS号:
9001-45-0
EC 号:
3.2.1.31 (BRENDA, IUBMB)
EC 号:
232-606-8
MDL编号:
MFCD00130629

重组

expressed in E. coli overproducing strain

质量水平

300

形式

lyophilized powder

比活

≥10,000,000 units/g protein (30 min assay)

分子量

~290 kDa

组成

protein, 40-60% biuret

储存温度

−20°C

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相关类别

应用

比较不同酶性能的新技术文章
了解更多
了解Sigma研发获得的最新应用数据，关于使用不同来源的酶优化不同药物类别的水解，并使用色谱纯化酶降低酯酶活性导致6-单乙酰吗啡(6-MAM)转化为吗啡的影响
有效水解类固醇葡糖苷酸。
用于尿液代谢物分析中葡糖苷酸结合物的水解

质量

含有缓冲盐和稳定剂。

单位定义

一个Sigma或改良的Fishman单元将在37℃，pH6.8（30分钟测定）下每小时从酚酞葡糖苷酸中释放1.0 μg酚酞。

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H317 - H334

预防措施声明

P261 - P280 - P342 + P311

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

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Enzymes
Methods of Enzymatic Analysis, 2, 460-461 (1974)
Ling Xiao et al.
RSC advances, 10(39), 22966-22971 (2020-06-16)
UDP-glucuronosyltransferases (UGTs) and β-glucuronidase (GUS) catalyze entirely distinct metabolism reactions. UGTs are responsible for the glucuronidation of a variety of drugs, endogenous and environmental chemicals, whereas GUS hydrolyzes glucuronides and liberates the parent substrates. Information on the overlap of ligand
Optimized conditions for the emzymatic hydrolysis of a-hydroxytriazolam-glucoronide in human urine.
Tsujikawa, K., et al.
Health Laboratory Science, 50, 286-289 (2004)
V Graef et al.
Clinical chemistry, 23(3), 532-535 (1977-03-01)
We determined the enzymic activity of beta-glucuronidase preparations from bovine liver, Helix pomatia, and Escherischia coli with steroid glucuronides and nonsteroid glucuronides as substrates. We also studied the effect of Na2SO4 on the enzymic hydrolysis of several substrates with the
G Cassinelli et al.
Biochemical pharmacology, 85(10), 1424-1432 (2013-03-08)
The activity of heparanase is responsible for heparan sulfate cleavage, thus resulting in the release of heparan sulfate-bound growth factors. Since heparanase activity is upregulated in several tumor types and is implicated in the malignant behavior, the enzyme is regarded
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商品

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics and Human Health

Today, diverse studies report the benefits of probiotics, such as inhibitory effects on pathogens, aid in the management or prevention of chronic intestinal inflammatory diseases or atopic syndromes, and support to the immune system. Potential beneficial applications abound, researchers continue to evaluate the effictiveness and clarify the mechanisms of action of probiotics.

Probiotics and Human Health

Today, diverse studies report the benefits of probiotics, such as inhibitory effects on pathogens, aid in the management or prevention of chronic intestinal inflammatory diseases or atopic syndromes, and support to the immune system. Potential beneficial applications abound, researchers continue to evaluate the effictiveness and clarify the mechanisms of action of probiotics.

实验方案

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Using UHPLC/MS (TOF) for Detection of Drugs and Metabolites in Urine Following Optimized Enzymatic Hydrolysis Conditions

To optimize hydrolysis using β-glucuronidase, factors such as incubation time, temperature, hydrolysis pH, enzyme source, and enzyme concentration must be evaluated for each glucuronide metabolite to be analyzed.

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