H9395
α-溶血素 来源于金黄色葡萄球菌
lyophilized powder, Protein ~60 % by Lowry, ≥10,000 units/mg protein
别名:
α-毒素
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关于此项目
Form:
lyophilized powder
Biological source:
Staphylococcus aureus
biological source
Staphylococcus aureus
Quality Level
form
lyophilized powder
specific activity
≥10,000 units/mg protein
contains
sodium citrate buffer as balance
composition
Protein, ~60% Lowry
solubility
H2O: soluble 0.49-0.51 mg/mL
UniProt accession no.
storage temp.
2-8°C
Gene Information
Staphylococcus aureus ... SAOUHSC_01121(3920722)
General description
α-溶血素是一种成孔细胞毒素，它是大多数致病性金黄色葡萄球菌菌株分泌的一种细胞外蛋白。它是以水溶性单体的形式分泌，是一种小型β桶状蛋白。
Application
α-来自金黄色葡萄球菌的溶血素已被用于:
- 作为电解质溶液的一种成分，用于通过电生理测量测试脂双层中的孔形成
- 测试其在骨髓基质细胞（BMSCs）中的成骨抑制作用
- 制备用于 Biacore 和表面等离子体共振的α溶血素分子印迹聚合物 (MIP)
α-溶血素被用于一项研究，用于测试乳制品大肠杆菌的外排泵和溶血素活性。它还用于测试其对苯扎氯铵的适应性以及环丙沙星对生物膜形成的影响。
Biochem/physiol Actions
α-溶血素是选择性溶血的，单体形式与膜结合，结合不需要特异性受体。在与生物膜和/或人造膜结合后，它会发生自身低聚化，从而产生代表跨膜孔的环状结构（六聚体），这些孔对离子和小代谢物是通透的。它对兔红细胞有明显的偏好。α-溶血素刺激细胞磷脂酶并诱导 Ca2+流入。它导致内皮屏障的膜破坏和细胞质成分的泄漏和细胞的渗透裂解。α-溶血素与脓毒症的发病机制有关。
Packaging
包装尺寸基于蛋白质含量
Other Notes
一个溶血单元是在 37°C 下 30 分钟，然后在 4°C 下冷藏 30 分钟后，悬浮在 pH 7.0 磷酸盐缓冲盐水中含1% 牛血清白蛋白的 1% 兔红细胞有50%溶解。
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2
target_organs
Lungs,Blood
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
高风险级别生物产品--毒素类产品
此项目有
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| H9395-1MG | 04061826738788 |
| H9395-.5MG | 04061833812648 |