Merck
CN

I3390

Sigma-Aldrich

Iscove 改良杜氏培养基

liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma

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别名:
IMDM
MDL编号:
NACRES:
NA.75

质量水平

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

phenol red: yes
L-glutamine: no
glucose: yes

运输

ambient

储存温度

2-8°C

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应用

Guilbert和Iscove证明红细胞和巨噬细胞的前体细胞可以在完全确定的无血清培养基中进行培养,该培养基需补充白蛋白、转铁蛋白、卵磷脂和硒。该培养基是Dulbecco′s改良Eagle′s培养基(DME)的改良版本,其含有硒、额外的氨基酸和维生素、丙酮酸钠、HEPES缓冲液、以及硝酸钾(代替硝酸铁)。进一步的研究表明,Iscove′s培养基可以支持小鼠B淋巴细胞、骨髓造血组织、脂多糖刺激的B细胞、T淋巴细胞和各种杂交细胞的成长。

重悬

以0.584 g/L补充 L-谷氨酰胺

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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N N Iscove et al.
The Journal of experimental medicine, 147(3), 923-933 (1978-03-01)
Albumin, transferrin, and lipids can replace serum entirely for support of LPS-stimulated murine B lymphocytes in culture. In the presence of these compounds, growth and maturation to IgM and IgG secretion, induced by lipopolysaccharide (LPS), occurs at the same or
Kirsten Gillingwater
Parasitology, 145(7), 953-960 (2017-11-24)
Trypanosoma equiperdum is a protozoan parasite responsible for causing Dourine, a debilitating neglected veterinary disease, found worldwide affecting equids. It is the only pathogenic trypanosome species that does not require an invertebrate vector for transmission, thus being passed from animal
S S Ozturk et al.
Journal of biotechnology, 18(1-2), 13-28 (1991-04-01)
A murine hybridoma cell line (167.4G5.3) was cultured in batch mode using IMDM containing different serum concentrations and bovine serum albumin (BSA). Cell growth and death, metabolism and antibody production were studied in these cultures. The cells were more susceptible
Complete replacement of serum in primary cultures of erythropoietin-dependent red cell precursors (CFU-E) by albumin, transferrin, iron, unsaturated fatty acid, lecithin and cholesterol.
N N Iscove et al.
Experimental cell research, 126(1), 121-126 (1980-03-01)
Yi Sun et al.
Human molecular genetics, 28(2), 245-257 (2018-10-03)
Rett syndrome (RTT) is a neurodevelopmental disorder caused by mutations or deletions in Methyl-CpG-binding Protein 2 (MeCP2), a brain-enriched transcriptional regulator. MeCP2 is highly expressed during neuronal maturation and its deficiency results in impaired dendritic morphogenesis and reduced dendritic spine

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