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Merck
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LUC1

荧光素酶报告子基因检测试剂盒

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关于此项目

NACRES:
NA.84
UNSPSC Code:
12352207
Usage:
 kit sufficient for 100 assays
Storage temp.:
−70°C
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usage

 kit sufficient for 100 assays

Quality Level

storage temp.

−70°C

General description

萤火虫荧光素酶是基因表达中最常用的报告基因之一。这是一种极度灵敏、响应快速且易于使用的报告基因。荧光素酶催化的化学发光反应是测量基因表达最敏感的分析工具之一。与传统方法相比,荧光素酶作用物含有辅酶 A,可增加和维持发光。这可避免向自动光度计进样底物,并允许通过摄影胶片或闪烁计数法进行分析。裂解液含有多粘菌素 B,用于进一步增强信号并消除溶菌酶处理和细胞冻融。细胞裂解液兼容β-半乳糖苷酶测定。由荧光素酶报告基因编码的酶在ATP、氧和Mg2+环境下催化D-萤光素的氧化。定量荧光产物以测定其活性。


pictograms

CorrosionEnvironment

signalword

Danger

存储类别

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1



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分析证书(COA)

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Firefly luciferase is a sensitive reporter for gene studies due to its absence in mammalian cells or tissues.


Gerben van Ooijen et al.
Current biology : CB, 21(10), 869-875 (2011-05-03)
Circadian clocks were, until recently, seen as a consequence of rhythmic transcription of clock components, directed by transcriptional/translational feedback loops (TTFLs). Oscillations of protein modification were then discovered in cyanobacteria. Canonical posttranslational signaling processes have known importance for clocks across
Esther Rincón et al.
Oncotarget, 8(28), 45415-45431 (2017-05-20)
Oncolytic virotherapy represents a promising alternative for cancer treatment; however, viral delivery to the tumor represents a major challenge. Mesenchymal stem cells (MSCs) chemotax to tumors, and can serve as a viral delivery tool. Previously, we demonstrated antitumor therapeutic efficacy
Rachel S Edgar et al.
Nature, 485(7399), 459-464 (2012-05-25)
Cellular life emerged ∼3.7 billion years ago. With scant exception, terrestrial organisms have evolved under predictable daily cycles owing to the Earth's rotation. The advantage conferred on organisms that anticipate such environmental cycles has driven the evolution of endogenous circadian rhythms



全球贸易项目编号

货号GTIN
LUC1-1KT04061833967263