产品名称
培养基 199, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
HEPES: no
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
NaHCO3: no
L-glutamine: yes
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
General description
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
Application
培养基199已用于：
- 测试和确定传感器在复杂环境中的pH灵敏度
- 作为卵母细胞采集的培养基
- 作为杜氏利什曼虫（LdBOB）原体培养基的组分，用于培养杜氏利什曼虫
- 内脏脂肪组织外植体
Preparation Note
按照每升培养基10.6克粉末配制。
补充0.35 g/L碳酸氢钠。
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存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.