Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes
shipped in
ambient
storage temp.
2-8°C
General description
最低必需培养基(MEM)是Harry Eagle开发的合成细胞培养基。培养基含有较高浓度的氨基酸，因此比较接近哺乳动物培养细胞的蛋白质成分。在含有Hanks′或Earle′s盐的配方中选择补充非必需氨基酸，可扩大该培养基的用途。混合Earle′s平衡盐的MEM是改良配方，又名αMEM，包含非必需氨基酸、丙酮酸钠和额外的维生素。
Application
Eagle最低必需培养基已用于：
- 自然杀伤细胞(NK)的维持培养
- 杜氏利什曼原虫(Leishmania donovani)菌株培养
- 大鼠毛囊隆突部位表皮神经嵴干细胞(EPI-NCSCs)的培养
Preparation Note
按照每升培养基10.1克粉末配制。
补充2.2 g/L碳酸氢钠。
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.