产品名称
培养基 199, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
shipped in
ambient
storage temp.
2-8°C
General description
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
Application
培养基199已用于：
- 培养人间皮细胞
- 作为保存培养基的组分，用于收集多产布尔山羊胚胎
- 制备球形包埋的胶原基质
Preparation Note
补充0.1 g/L L-谷氨酰胺，2.2 g/L碳酸氢钠（1×）。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
Elena García-Gareta et al.
BioResearch open access, 2(6), 412-420 (2014-01-01)
The clinical demand for effective dermal substitutes continues as current commercially available products present limitations. However, there are no definitive in vitro methods to predict in vivo outcomes such as integration, cellularization and contraction, which may help the development of
Chiemi Toyama-Mori et al.
Cryobiology, 88, 92-97 (2019-03-14)
Effects of additional physical treatments during vitrification of the bovine ovarian tissue were examined for increasing of permeability of ethylene glycol (EG) and dimethyl sulfoxide (Me2SO). The concentrations of EG and Me2SO and histological changes in the ovarian tissue were
Woo-Jin Shin et al.
mBio, 9(6) (2018-11-30)
As oral poliovirus vaccine (OPV) causes vaccine-associated paralytic poliomyelitis, the polio endgame strategy introduced by the Global Polio Eradication Initiative calls for a phased withdrawal of OPV and an introduction of inactivated poliovirus vaccine (IPV). The introduction of IPV creates
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| M0650-100ML | 04061833993811 |