产品名称
Eagle最低必需培养基, With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
phenol red: yes
NaHCO3: no
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
General description
它是经典MEM配方的改良版。厄尔′盐类已被汉克′盐类所取代。由于碳酸氢盐浓度显著降低，因此该介质具有显著降低的缓冲能力。含有19个氨基酸。必需氨基酸和非必需氨基酸；L-ala；L-asn；L-asp；L-glu；L-gly；L-pro和L-ser。可优先用于在减少的血清存在下的贴壁细胞的生长或作为开发无血清培养基的基础。
Preparation Note
按照每升培养基10.7克粉末配制。
补充0.35 g/L碳酸氢钠。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
商品
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.