Merck
CN

M2279

Sigma-Aldrich

Eagle最低必需培养基

With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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别名:
MEM
NACRES:
NA.75

质量水平

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

运输

ambient

储存温度

2-8°C

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一般描述

最低必需培养基(MEM)是使用最广泛的合成细胞培养基之一。它包含更高浓度的氨基酸,所以培养基更接近培养哺乳动物细胞的蛋白质组成。MEM已用于在胎牛血清(FBS)、小牛或马血清环境中培养各种单层细胞和附着细胞系(如成纤维细胞)。

应用

最低必需培养基Eagle已用于培养:
  • 主动脉瓣小叶的外植体
  • 人肝癌细胞系(HepG2)细胞
  • 来源于人胎儿大脑祖细胞的星形胶质细胞(PDA)

制备说明

补充0.292 g/L L-谷氨酰胺。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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