Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM
shipped in
ambient
storage temp.
2-8°C
General description
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
Application
培养基199已用于：
- 在成熟前保持卵丘-卵母细胞复合体（COC），并培养这些卵母细胞在体外成熟
- 在德国荷斯坦奶牛卵子采集过程中清洗抽吸管
- 培养大鼠心脏内皮细胞
Preparation Note
补充 2.2 g/L 碳酸氢钠。
配制成每升培养基含 14.7 克粉末。
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
粉末培养基及盐混合物极具吸湿性，应注意防潮。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
商品
钙是一种离子稳定的二价阳离子，在细胞培养基中具有有益和有毒的双重属性，可作为一系列细胞培养基的组成部分。
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.