Merck
CN

M4530

Sigma-Aldrich

培养基 199

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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别名:
M199 Medium, TCM 199
NACRES:
NA.75

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

NaHCO3: yes
L-glutamine: yes
HEPES: no
Earle’s salts (5% CO2): yes
phenol red: yes
sodium pyruvate: no

运输

ambient

储存温度

2-8°C

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一般描述

培养基199是维生素、氨基酸和其他因素的组合,最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性,特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
按照 Morgan 公布的原始 M 199 配方配制的完全液体培养基。

应用

培养基199已用于:
  • 作为悬浮培养基维持原代神经元细胞生长
  • 作为胶原蛋白凝胶组分离体培养胚心
  • 在慢速冷冻处理中,维持卵巢组织切片活性

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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