Merck
CN

M5650

Sigma-Aldrich

Eagle最低必需培养基

With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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别名:
MEM
NACRES:
NA.75

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

运输

ambient

储存温度

2-8°C

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一般描述

Eagle最低必需培养基(MEM)是Harry Eagle开发的人工合成细胞培养基。培养基含有较高浓度的氨基酸,因此比较接近哺乳动物培养细胞的蛋白质成分。在含有Hanks′或Earle′s盐的配方中选择补充非必需氨基酸,可扩大该培养基的用途。通过选择性去除钙成分后,该配方可进一步改良为适合悬浮细胞培养。MEM可用于各种细胞的单层培养。

应用

Eagle最低必需培养基已用于培养:
  • HeLa细胞
  • Madin-Darby犬肾细胞 (MDCK)
  • 软骨肉瘤细胞系

此培养基在血清减量条件下适合贴壁细胞生长,也可作为无血清培养基开发的基础配方。

重悬

添加 0.292g/L L-谷氨酰胺。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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