Merck
CN

M7167

Sigma-Aldrich

M2 培养基

With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

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MDL编号:
NACRES:
NA.75

无菌性

sterile-filtered

形式

liquid

储存条件

protect from light

technique(s)

cell culture | embryo: suitable

杂质

endotoxin, tested

组分

HEPES: 5.42726 g/L
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L

运输

ambient

储存温度

2-8°C

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应用

推荐用于室温下操作小鼠胚胎。M2 和 M16 培养基是植入前期胚胎体外培养的常用培养基。它是一种改良的Krebs-Ringer碳酸氢盐溶液,与Whitten′s培养基非常相似。M16含有丙酮酸和乳酸作为能量来源,因为植入前胚胎不能有效利用葡萄糖。

M2培养基是对M16的进一步改良,用HEPES缓冲液替代一些碳酸氢盐。M2可用于在CO2培养箱外长时间收集和处理胚胎。

质量

溶液中的沉淀不影响本品性能。

重悬

添加 0.06g/L 青霉素 G 钾盐和 0.05g/L 硫酸链霉素。

分析说明

该产品经过测试,能够支持单细胞小鼠胚胎发育成扩大的胚泡。对胚泡发育的最低要求是 80%。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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Jane C Fenelon et al.
Biology of reproduction, 97(1), 119-132 (2017-06-24)
Embryonic diapause is a common reproductive strategy amongst mammals, requiring an intimate cross-talk between the endometrium and the blastocyst. To date, the precise molecular signals responsible are unknown in the mouse or any other mammal. Previous studies in the mink
Hongzheng Sun et al.
Biology of reproduction, 100(3), 601-617 (2018-09-29)
Methionine adenosyltransferase II (MAT2A) is essential to the synthesis of S-adenosylmethionine, a major methyl donor, from L-methionine and ATP. Upon fertilization, zygotic genome activation (ZGA) marks the period that transforms the genome from transcriptional quiescence to robust transcriptional activity. During
Yixin Xu et al.
Gene expression patterns : GEP, 25-26, 46-58 (2017-06-07)
Cumulus cells (CCs) are considered as an important source to predict oocyte quality. Despite numerous candidate genes in CCs have been identified for embryonic developmental competence, the results are inconsistent. The next generation RNA-sequencing was used to investigate the transcriptomic
Sarah E M Stephenson et al.
Scientific reports, 8(1), 7528-7528 (2018-05-16)
Mutations in PARK2 (parkin) can result in Parkinson's disease (PD). Parkin shares a bidirectional promoter with parkin coregulated gene (PACRG) and the transcriptional start sites are separated by only ~200 bp. Bidirectionally regulated genes have been shown to function in common
Lanping Hao et al.
Biology of reproduction, 80(5), 944-953 (2009-01-09)
DDK syndrome is the polar-lethal embryonic death that occurs at the morula-blastocyst transition when female mice of the DDK strain are mated with males from many other inbred strains (so-called alien males). Embryonic death is caused by incompatibility between a

实验方案

Creating Transgenic Mice using CRISPR-Cas9 Genome Editing

利用 CRISPR-Cas9 基因组编辑技术创建转基因小鼠

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