膜支架蛋白 1E3D1

Research area: Cell Struc

Nanodiscs are non-covalent structures that contain a phospholipid bilayer and a membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1). The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E3D1 variant of MSP1 differs from MSP1 in the following facets: It deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence³ (which is known separately as MSP1D1). It repeats the Helix 4 (H4), Helix 5 (H5) and Helix 6 (H6) sequences of the original MSP1 sequence between the parent Helix 6 (H6) and Helix 7 (H7) segments of MSP1D1.

纳米盘可溶性脂质双层系统已被证明是一种广泛适用的手段，用于使膜蛋白溶在水溶液中的类似天然的双层膜环境中，维持其单分散和活性。纳米盘的关键组分是包绕着两亲性螺旋蛋白质带（膜支架蛋白）。

纳米盘系统已被用于包裹多种蛋白质，包括 GPCR、P450、细菌视紫红质、凝血因子、霍乱毒素、TAR 受体和芳香酶。

要获取关于引用和实验方案的详细列表，请访问 Sligar Lab 网网站 sligarlab.life.uiuc.edu/nanodisc.html

For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.

生成直径约 12.9nm 的纳米盘

纳米盘技术，以及它的许多用途，均由伊利诺伊大学所有的下列专利所保护。

• 7,691,414 膜支架蛋白
• 7,662,410 膜支架蛋白和嵌入膜蛋白
• 7,622,437组织因子组合物和方法
• 7,592,008 膜支架蛋白
• 7,575,763 膜支架蛋白和栓系膜蛋白
• 7,083,958 膜支架蛋白
• 7,048,949 膜支架蛋白

Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.

• 7,691,414 Membrane scaffold proteins
• 7,662,410 Membrane scaffold proteins and embedded membrane proteins
• 7,622,437 Tissue factor compositions and methods
• 7,592,008 Membrane scaffold proteins
• 7,575,763 Membrane scaffold proteins and tethered membrane proteins
• 7,083,958 Membrane scaffold proteins
• 7,048,949 Membrane scaffold proteins