Merck
CN

N2770

Sigma-Aldrich

SIGMAFAST 对硝基苯基磷酸酯片

tablet, To prepare 20 mL

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别名:
pNPP tablets
EC 号:
MDL编号:
NACRES:
NA.83

形式

tablet

溶解性

water: 0.25 tablet/mL, clear, colorless

储存温度

−20°C

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应用

开发用作酶免疫分析 (EIA) 中检测碱性磷酸酶活性的可溶性底物。pNPP 是基于高灵敏度碱性磷酸酶的 EIA 检测中的首选底物。将 1 片 pNPP 片剂和 1 片 Tris 缓冲液片剂溶于去离子水中可作为即用型底物。各 Sigma 快速 pNPP 片剂组得到的溶液含 1.0 mg/mL pNPP,0.2 MTris 缓冲液。使用 pNPP 的 EIA 应用可在定时测定中读取,或用 3N NaOH 停止以延迟读取。SIGMA 快速 pNPP仅需要添加蒸馏水或去离子水,即可制备活性底物溶液。片剂单独包装在铝箔包装中 。

重悬

将每片片剂溶于 20 mL 去离子水中,得到含 pNPP 的即用型缓冲液。

法律信息

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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示例

T1503
货号
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25G
包装规格/数量

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

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Yau-Hung Chen et al.
International journal of biological macromolecules, 126, 159-169 (2018-12-27)
To fulfill the properties of membrane for guided bone tissue regeneration, chitosan (CS) and calcium phosphates were blended to produce porous hybrid membranes by lyophilization. We synthesized three different calcium phosphates: calcium deficient hydroxyapatite (CDHA), biphasic calcium phosphate (BCP) and
G Gasperini et al.
Infection and immunity, 89(4) (2020-12-16)
Typhoid and paratyphoid fevers have a high incidence worldwide and coexist in many geographical areas, especially in low-middle-income countries (LMIC) in South and Southeast Asia. There is extensive consensus on the urgent need for better and affordable vaccines against systemic
Farhana Haque et al.
Acta neuropathologica communications, 5(1), 45-45 (2017-06-08)
Missense somatic mutations affecting histone H3.1 and H3.3 proteins are now accepted as the hallmark of paediatric diffuse intrinsic pontine gliomas (DIPG), non-brain stem paediatric high grade gliomas (pHGG) as well as a subset of adult glioblastoma multiforme (GBM). Different
Fernando Diaz et al.
Virology, 513, 129-135 (2017-10-27)
Herpes simplex virus 2 (HSV-2) is the leading cause of genital herpes and increases the risk of HIV infection, but there is no effective vaccine. A replication-defective HSV-2 mutant virus, dl5-29, is effective in animal models and has been in
Zhongyuan Guo et al.
Nanotoxicology, 12(5), 485-508 (2018-04-19)
The use of nanomaterials to enhance properties of food and improve delivery of orally administered drugs has become common, but the potential health effects of these ingested nanomaterials remain unknown. The goal of this study is to characterize the properties

商品

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

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