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安全信息

NA2010

Sigma-Aldrich

GenElute™ 血液基因组DNA试剂盒

sufficient for 70 purifications

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别名:
血液基因组DNA, Gen Elute
NACRES:
NA.55

用途

sufficient for 70 purifications

储存温度

15-25°C
room temp

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相关类别

一般描述

GenElute血液基因组DNA试剂盒可以简单、方便地从新鲜或长时间存放（超过24小时）的全血中分离高纯度基因组DNA。该试剂盒将硅胶膜技术的优势与微量离心形式结合在一起，同时无需采用昂贵的树脂、乙醇沉淀，以及诸如苯酚和氯仿等有害化学成分。

应用

GenElute™血液基因组DNA试剂盒已被用于：
  • 从切除输精管的兔子体内获得的大颗粒（LG）和小颗粒（SG）样品中提取DNA
  • 从血液样本中分离DNA
  • 从剩余血细胞中提取全血DNA

纯化所得的基因组DNA可用于以下下游应用：
  • 限制性内切酶酶切
  • PCR
  • Southern印迹
  • 测序反应
  • 克隆

特点和优势

  • 起始材料：不超过200 μl新鲜或长时间存放的血液
  • 预期得率：至多10 μg
  • 洗脱体积：400 μl
  • 所需时间：<40分钟
  • A260/A280比率：1.6 - 1.9
  • 兼容多种抗凝血素、包括EDTA、肝素和柠檬酸钠

原理

起始材料首先溶解在含有离液盐的溶液之中，以确保大分子彻底变性。之后加入乙醇，从而使DNA在溶解物通过微量离心管在硅胶膜上离心时，结合到膜上。随附一份Prewash Solution，可帮助与全血样本长时间存放（超过24小时）相关的污染物。在洗涤去除污染物后，将DNA放在200 mL的Tris-EDTA溶液中洗脱。

预期基因组DNA得率取决于所用的起始材料的数量和性质（例如，从200 μl 新鲜的全血之中，可在一小时内分离出4至10 μg RNase A处理的DNA）。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。

其他说明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • C2112Column Preparation Solution化学品安全说明书
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology化学品安全说明书
  • R6148RNase A solution化学品安全说明书

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警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 3

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实验方案

Whole Genome Amplification from Serum or Plasma Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

Whole Genome Amplification from Serum or Plasma Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

Extraction & Amplification of Whole Blood Using WGA-Protocol

Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged whole blood products. Once the DNA is isolated, it can be amplified using the GenomePlex® Whole Genome Amplification protocol.

Extraction & Amplification of Whole Blood Using WGA-Protocol

Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield. This protocol is a simple method to isolate DNA from fresh or aged whole blood products. Once the DNA is isolated, it can be amplified using the GenomePlex® Whole Genome Amplification protocol.

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