Merck
CN
所有图片(2)

文件

安全信息

P0982

Sigma-Aldrich

JumpStart™ REDTaq® ReadyMix™ 反应混合物

for PCR

登录查看公司和协议定价
所有图片(2)
别名:
Hot start PCR master mix, Hot start master mix, Hot start taq master mix
MDL编号:
MFCD00166026

质量水平

200

形式

liquid

用途

sufficient for 100 reactions
 sufficient for 20 reactions
 sufficient for 800 reactions

特点

dNTPs included
hotstart

浓度

2.5 units/reaction (50 μL reaction volume)

技术

PCR: suitable

颜色

red

输入

purified DNA

应用

agriculture

运输

wet ice

储存温度

−20°C

正在寻找类似产品? Visit 产品对比指南

相关类别

一般描述

JumpStart REDTaq ReadyMix PCR反应混合物是制备好的溶液，结合了热启动PCR的性能优势和Sigma ReadyMix和REDTaq的双重便利性。反应混合物优化的2x反应浓缩物中包括Sigma的JumpStart Taq DNA聚合酶、99％纯度的脱氧核苷酸、缓冲液和惰性红色染料。将25 μL ReadyMix加到引物、模板和水中，使最终反应体积为50 μL。在室温下，反应混合物中的JumpStart Taq抗体可使Taq DNA聚合酶失活。在PCR的第一次变性步骤期间，复合物解离，聚合酶活性完全恢复。惰性红色染料不影响自动测序、限制性酶切、连接或其他下游操作。如果需要，只需通过标准纯化方法就可将PCR产物与染料轻松分离。热启动机制允许设置室温，使其成为高通量应用的理想选择 。

应用

Jumpstart™ REDTaq® ReadyMix™反应混合物可用于直接提取DNA并进行PCR扩增。还用于：
  • 多重定量PCR
  • 甲基化特异性PCR(MSPCR)
  • 总RNA合成cDNA的PCR扩增

特点和优势

  • JumpStart™ REDTaq® DNA聚合酶是一种抗体失活的热启动酶，旨在最大限度地减少非特异性扩增，同时提高目标产量和特异性
  • 无论模板浓度如何，JumpStart Taq DNA聚合酶均可提供优越的扩增效果
  • REDTaq JumpStart ReadyMix可减少移液步骤，降低污染风险。 热启动机制允许设置室温，使其成为高通量应用的理想选择 。
  • 惰性红色染料方便验证试剂是否适当混合
  • 不需要装载其他染料。可以在反应后将等分试样直接加载到琼脂糖凝胶上

包装

默认的反应体积为50 μL

20RXN包装成1 X 500 μL
100RXN包装成1 X 2.5 mL
800RXN包装成1 X 20 mL

单位定义

在74℃下，一个单位可在30分钟内将10 nM总dNTP掺入酸可沉淀的DNA中。

其他说明

想查看JumpStart REDTaq和ReadyMix产品的更多详细信息，请访问 www.sigma-aldrich.com/hotstart。
对于典型的PCR反应，将25 μL JumpStart™ REDTaq® ReadyMix™反应混合物与25 μL混合物（含有模板DNA、引物和水）混合。可根据需要改变反应体积。

法律信息

凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权（如，5′ Nuclease Process专利权）。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：US 8,404,464和US 7,972,828。 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可。
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

法规信息

常规特殊物品
含少量动物源组分生物产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

为方便起见，与您过往购买产品相关的文件已保存在文档库中。

访问文档库

难以找到您所需的产品或批次号码？

在网站页面上，产品编号会附带包装尺寸/数量一起显示（例如：T1503-25G）。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题？欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ （Lot或Batch）字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。

  • 如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。

  • 如果您查询到的批号含有填充代码（例如 STBB0728K9），请输入去除填充代码K9的批号：STBB0728。

未找到您寻找的产品？

部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。

索取COA

Nijiro Nohata et al.
Developmental biology, 411(2), 183-194 (2016-02-14)
Angiogenesis, the formation of new blood vessels by remodeling and growth of pre-existing vessels, is a highly orchestrated process that requires a tight balance between pro-angiogenic and anti-angiogenic factors and the integration of their corresponding signaling networks. The family of
Stephen J Murphy et al.
DNA research : an international journal for rapid publication of reports on genes and genomes, 19(5), 395-406 (2012-09-20)
High-throughput next-generation sequencing provides a revolutionary platform to unravel the precise DNA aberrations concealed within subgroups of tumour cells. However, in many instances, the limited number of cells makes the application of this technology in tumour heterogeneity studies a challenge.
Stephen J Murphy et al.
Gastroenterology, 145(5), 1098-1109 (2013-08-06)
Increasing grade of pancreatic intraepithelial neoplasia (PanIN) has been associated with progression to pancreatic ductal adenocarcinoma (PDAC). However, the mechanisms that control progression from PanINs to PDAC are not well understood. We investigated the genetic alterations involved in this process. Genomic
Wendy Monger et al.
Archives of virology, 163(6), 1585-1594 (2018-03-02)
A novel virus was discovered in a freeze-dried collection held at SASA, UK, originating from potato (Solanum tuberosum) cv. Nadine. The complete sequence of the viral RNA was determined to be 3674 nucleotides in length encoding five predicted proteins. Based
Tatiana V Danilova et al.
Chromosoma, 121(6), 597-611 (2012-10-12)
Fluorescence in situ hybridization (FISH) is a useful tool for physical mapping of chromosomes and studying evolutionary chromosome rearrangements. Here we report a robust method for single-copy gene FISH for wheat. FISH probes were developed from cDNA of cytosolic acetyl-CoA
查看所有相关文献

商品

Qualitative multiplex PCR assay for assessing DNA quality from FFPE tissues and other sources of damaged DNA

The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis, LOH, aCGH, etc.).

Qualitative multiplex PCR assay for assessing DNA quality from FFPE tissues and other sources of damaged DNA

The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis, LOH, aCGH, etc.).

Introduction and Historical Timelines

Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

查看所有结果

实验方案

REDTaq™应用

介绍RedTaq的应用和优势，包括用于基因组DNA PCR的标准RedTaq、热启动RedTaq和RedTaq。

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门