P2088
过氧化物酶 来源于辣根
Highly stabilized, essentially salt-free, lyophilized powder, 200-300 units/mg solid (using pyrogallol)
别名:
供体：过氧化氢氧化还原酶, 辣根过氧化物酶
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关于此项目
化学文摘社编号:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-668-6
MDL number:
Specific activity:
200-300 units/mg solid (using pyrogallol)
Biological source:
horseradish
biological source
horseradish
Quality Level
form
essentially salt-free, lyophilized powder
specific activity
200-300 units/mg solid (using pyrogallol)
mol wt
~44 kDa
absorbance ratio
RZ 2.6-3.4
storage temp.
2-8°C
SMILES string
[O+H2]O[O-]
InChI
1S/H2O3/c1-3-2/h1-2H
InChI key
JSPLKZUTYZBBKA-UHFFFAOYSA-N
General description
辣根过氧化物酶是从辣根（Amoracia rusticana）中分离出来的，属于过氧化物酶的铁原卟啉基团。HRP是含有四个二硫键的单链多肽。它是一种含有18%碳水化合物的糖蛋白。碳水化合物由半乳糖、阿拉伯糖、木糖、岩藻糖、甘露糖、甘露糖胺和半乳糖胺组成，具体取决于特定同工酶。其分子量（约44 kDa）包括多肽链（33,890道尔顿）、氯化血红素加Ca2+ （约700道尔顿）和碳水化合物（约9,400道尔顿）。至少存在7种HRP同工酶。 辣根过氧化物酶同工酶的等电点范围为3.0-9.0。
Application
辣根过氧化物酶已被用于脱氢聚合物 (DHP) 的研究，以研究单寡聚物偶联机制。 它还被用来通过创建辣根过氧化物酶模拟DNAzyme来帮助开发用于microRNA（miRNA）检测的比色方法。
Biochem/physiol Actions
已证明辣根过氧化物酶可催化多种物质的聚合，包括对乙酰氨基酚和腰果酚。
Preparation Note
通过伯胺的化学保护而保持稳定。
Analysis Note
RZ(Reinheitszahl)是指溶于去离子水中的酶(0.5–1.0 mg/ml)的吸光度比值A403/A275 。 它是血红素含量的量度，而不是酶活性。 即使是RZ值较高的制剂也可能具有较低的酶活性。
Other Notes
一个连苯三酚单元将在20℃、pH 6.0条件下，在20秒内从连苯三酚形成1.0mg的红棓酚。
有关过氧化物酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer。
Disclaimer
在低 pH 值和较高温度下保持活性。
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
实验方案
该实验程序用于使用连苯三酚作为底物测定过氧化物酶的酶活性。
建立以 2,2'-连氮基-双（3-乙基苯并噻唑啉-6-磺酸）为基质的过氧化物酶测定方法。
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
商品
Discover our peroxidase from horseradish enzymes, products, substrates, and inhibitors for your ELISA, immunoassay, and protein application needs.
D W Potter et al.
Molecular pharmacology, 29(2), 155-162 (1986-02-01)
Horseradish peroxidase catalyzed the polymerization of acetaminophen. Addition of reduced glutathione (GSH) to reaction mixtures resulted in decreased polymerization and formation of minor amounts of GSH-acetaminophen conjugates. The conjugates were identified as 3-(glutathion-S-yl)acetaminophen and 3-(glutathion-S-yl)diacetaminophen. Horseradish peroxidase also catalyzed polymerization
Keehoon Won et al.
Biomacromolecules, 5(1), 1-4 (2004-01-13)
Horseradish peroxidase-catalyzed polymerization of cardanol in aqueous organic solvent was investigated in the presence of a redox mediator. Cardanol is a phenol derivative from a renewable resource mainly having a C15 unsaturated hydrocarbon chain with mostly 1-3 double bonds at
Yanqin Wen et al.
Analytical chemistry, 84(18), 7664-7669 (2012-08-30)
We present a highly sensitive colorimetric method for microRNA (miRNA) detection. This method is based on a rolling-circle amplification (RCA) DNA machine, which integrates RCA, nicking enzyme signal amplification and DNAzyme signal amplification. The DNA machine is triggered by the
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P2088-10KU | 04061832731070 |
| P2088-5KU | 04061834357810 |