Quality Level
form
liquid
packaging
vial of 1.5 mL
color
colorless
application(s)
agriculture
foreign activity
DNase, RNase, none detected
storage temp.
−20°C
Application
10X PCR缓冲液已用作PCR混合物组分：
- 用于病毒DNA扩增
- 扩增基因组DNA，研究注意缺陷多动障碍(ADHD)患者的亚甲基四氢叶酸还原酶(MTHFR)基因突变
- 扩增芽孢杆菌来源的16S-23S rRNA基因内转录间隔区(ITS)
Features and Benefits
- 独立缓冲液
- 可与JumpStart™ Taq DNA聚合酶（D9307）、来自栖热水生菌的Taq DNA聚合酶 （D1806）和REDTaq® 基因组DNA聚合酶（D8312）兼容
Preparation Note
10×缓冲液的组成：100mM Tris-HCl（pH8.3，25℃）；500mM KCl；15mM MgCl2；0.01％明胶
Legal Information
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
新产品
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实验方案
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.