Merck
CN

P7998

Sigma-Aldrich

用于ELISA的碱性磷酸酶黄色(pNPP)液体底物系统

ready to use solution

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别名:
Alkaline phosphatase substrate solution (pNPP)
eCl@ss:
42010105
NACRES:
NA.83

质量水平

形式

liquid

储存温度

2-8°C

一般描述

本品是由 硝基苯基磷酸酯(pNPP)组成的缓冲碱性磷酸酶底物。这个先进的基质系统含有不溶性稳定剂,可提高稳定性和反应性,并降低基线吸收。它产生一种可溶性终产物,因此不推荐用于蛋白质印迹或组织化学应用。

应用

产生一种可溶性黄色反应产物,可在405nm处读取。对于终点测定,可以使用3N NaOH终止反应。推荐用于ELISA(微孔)分析;不建议用于膜应用。

注意

 可在2-8°C下稳定保存至少一年。

外形

即用型

制备说明

含有对硝基苯基磷酸酯,它是碱性磷酸酶的底物。

象形图

CorrosionHealth hazard

警示用语:

Danger

危险声明

危险分类

Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Laura C Jacques et al.
Nature communications, 11(1), 1892-1892 (2020-04-22)
Streptococcus pneumoniae serotype 1 is the predominant cause of invasive pneumococcal disease in sub-Saharan Africa, but the mechanism behind its increased invasiveness is not well understood. Here, we use mouse models of lung infection to identify virulence factors associated with
Nuttada Panpradist et al.
PloS one, 11(1), e0145962-e0145962 (2016-01-12)
Human immunodeficiency virus (HIV) is a chronic infection that can be managed by antiretroviral treatment (ART). However, periods of suboptimal viral suppression during lifelong ART can select for HIV drug resistant (DR) variants. Transmission of drug resistant virus can lessen
Hannah M Rowe et al.
Infection and immunity, 87(10) (2019-07-17)
Acute otitis media is one of the most common childhood infections worldwide. Currently licensed vaccines against the common otopathogen Streptococcus pneumoniae target the bacterial capsular polysaccharide and confer no protection against nonencapsulated strains or capsular types outside vaccine coverage. Mucosal
Cristina A Teixeira et al.
Clinical science (London, England : 1979), 130(18), 1665-1675 (2016-07-08)
Transthyretin (TTR)-related amyloidoses are diseases characterized by extracellular deposition of amyloid fibrils and aggregates in tissues composed of insoluble misfolded TTR that becomes toxic. Previous studies have demonstrated the ability of small compounds in preventing and reversing TTR V30M deposition
Molhm Nassir et al.
Organic & biomolecular chemistry, 17(46), 9913-9923 (2019-11-14)
Nucleotide pyrophosphatase/phosphodiesterase-1 (NPP1) inhibitors have been suggested as a potential treatment for calcium pyrophosphate dihydrate (CPPD) deposition disease. Here, we targeted the development of improved NPP1 inhibitors based on acyclic mimics of Pα,α-phosphorodithioate-substituted adenine nucleotides, 7-10. The latter were obtained

商品

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

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