Quality Level
form
liquid
usage
sufficient for 50 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s PCR 50-2,000 bp标记包含8个dsDNA片段,间隔50-2,000个碱基,间隔均匀。 可以将5μl梯子直接装在琼脂糖或聚丙烯酰胺凝胶上的单个泳道中。
Application
PCR 50 - 2,000 bp标记物可用作DNA凝胶电泳的标记物。
Other Notes
Sigma′s PCR 50 - 2,000 bp标记物为两个试管:
- 1瓶(250 μl体积)PCR标记物
- 1瓶6×上样缓冲液(250 μl体积),用于稀释样品
为获得最佳分离度,推荐琼脂糖凝胶浓度为 2.0%。
在可预测的间隔(阶梯)双链DNA片段中包含8个条带,50-2,000 bp。
存储类别
10 - Combustible liquids
ppe
Eyeshields, Gloves
法规信息
常规特殊物品
此项目有
实验方案
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
商品
选择适合的标记物和分子量标准,用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.