Merck
CN

PF0100

Sigma-Aldrich

蛋白酶检测试剂盒(荧光法)

high sensitivity assay

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NACRES:
NA.26

运输

dry ice

储存温度

−20°C

应用

蛋白酶荧光检测试剂盒已用于:
  • 通过基于酪蛋白的蛋白酶测定,检测蛋白水解活性
  • 检测真菌菌株的细胞外蛋白水解活性
  • 定量线粒体蛋白酶活性
  • 测定土壤溶液中的蛋白酶生成

适用性

检测限:最大50ng胰蛋白酶

数量

提供了足以用于200次一毫升反应体系的试剂。
提供了足以用于200次一毫升反应体系的试剂。

三氯乙酸(TCA)组分以6.1 N TCA 溶液的形式提供。0.6 N TC A溶液可通过将所需体积的 T0699 用超纯水进行10倍稀释而制备。使用试剂盒时无需将所有体积的 T0699 均进行稀释。

分析说明

已测试过其在全部4种蛋白酶水解类别中的检测效果(丝氨酸、天冬氨酸、半胱氨酸和金属蛋白酶)。

仅试剂盒组分

产品编号
说明

  • Incubation buffer 25 mL

  • FITC-casein solution 5 mL

  • Assay buffer 200 mL

  • Protease standard (trypsin) 20 μg

  • FITC control 50 mg

  • Trichloroacetic acid (TCA) solution 30 mL

警示用语:

Danger

危险分类

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

靶器官

Respiratory system

储存分类代码

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Selena Gimenez-Ibanez et al.
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Pathogenicity of Pseudomonas syringae is dependent on a type III secretion system, which secretes a suite of virulence effector proteins into the host cytoplasm, and the production of a number of toxins such as coronatine (COR), which is a mimic
M Lee et al.
Neuroscience, 229, 164-175 (2012-10-27)
Astrocytes become activated in degenerative neurological diseases. In order to gain a greater understanding of the inflammatory factors released upon activation, we stimulated adult human astrocytes with interferon-gamma and examined the resultant conditioned medium (CM) for toxicity against differentiated human
Fungal contamination of textile objects preserved in Slovene museums and religious institutions
Kavkler K, et al.
International Biodeterioration & Biodegradation, 97, 51-59 (2015)
Marion F Cubitt et al.
Molecular plant-microbe interactions : MPMI, 26(3), 356-366 (2012-11-02)
Plant cell wall-degrading enzymes (PCWDE) are key virulence determinants in the pathogenesis of the potato pathogen Pectobacterium atrosepticum. In this study, we report the impact on virulence of a transposon insertion mutation in the metJ gene that codes for the

实验方案

The Protease Fluorescent Detection Kit provides ready-to-use reagents for detecting the presence of protease activity. This simple assay to detect protease activity uses casein labeled with fluorescein isothiocyanate (FITC) as the substrate.

The Protease Fluorescent Detection Kit provides ready-to-use reagents for detecting the presence of protease activity. This simple assay to detect protease activity uses casein labeled with fluorescein isothiocyanate (FITC) as the substrate.

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