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安全信息

R0883

Sigma-Aldrich

RPMI-1640 培养基

With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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别名:
Roswell Park Memorial Institute 1640 medium
MDL编号:
MFCD00217820
NACRES:
NA.75

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成，用于在悬浮培养中支持淋巴母细胞生长，但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用，但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。

应用

RPMI-1640培养基已用于：
  • 培养PC-3前列腺细胞系和白血病细胞
  • 维持三阴性MDA-MB-231和管腔型MCF7白种人乳腺癌细胞系
  • 培养循环肿瘤细胞（CTC）

重悬

补充 0.3 g/L L-谷氨酰胺。

补充剂

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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