R6265
Eco R I 来源于大肠杆菌 BS5
Restriction Enzyme
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.53
EC Number:
279-487-9
MDL number:
Concentration:
10,000 units/mL
grade
Molecular Biology
Quality Level
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
Eco RI 是一种限制性内切酶，在分子生物学应用中用于切割识别站点 5′ 的 DNA-G/AATTC-3′，产生带有 5′ 的片段-粘性终点。
Biochem/physiol Actions
识别序列： 5′-G/AATTC-3′
切割结果：1 μgλ DNA 底物的 2-10 倍 EcoR I 过度消化导致 100% 切割
热灭活： 65°C，20 min。
切割结果：1 μgλ DNA 底物的 2-10 倍 EcoR I 过度消化导致 100% 切割
热灭活： 65°C，20 min。
Physical form
10 mM Tris-HCl 溶液，pH 值 7.2、1 mM EDTA、200 mM NaCl、0.5 mM 二硫赤藓糖醇、50% 甘油 (v/v)、0.2%Triton X-100 (v/v)，4°C
Other Notes
备注： 避免次优反应条件，如低盐、高 pH 值 ( 8.0) 和高甘油 ( 5%)，因为它们将改变 EcoRI 特异性并沉淀星号活性。
一个单位表示在37°C下1小时内，在总体积为50 ml的1x限制酶消化缓冲液SH中完全切割1 mg λDNA的酶活性。1 mg pBR322 DNA可通过2个单位的EcoR I完全消化。
提供 10x 限制性内切酶缓冲液 SH (B3657)。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
J Hedgpeth et al.
Proceedings of the National Academy of Sciences of the United States of America, 69(11), 3448-3452 (1972-11-01)
The sequence of DNA base pairs adjacent to the phosphodiester bonds cleaved by the RI restriction endonuclease in unmodified DNA from coliphage lambda has been determined. The 5'-terminal nucleotide labeled with (32)P and oligonucleotides up to the heptamer were analyzed
Annabel A Ferguson et al.
Methods in molecular biology (Clifton, N.J.), 940, 87-102 (2012-10-30)
The generation of transgenic animals is an essential part of research in Caenorhabditis elegans. One technique for the generation of these animals is biolistic bombardment involving the use of DNA-coated microparticles. To facilitate the identification of transgenic animals within a
Cong Zhu et al.
Nucleic acids research, 41(4), 2455-2465 (2013-01-11)
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs for many genomic sequences using publicly available zinc-finger modules. This limitation is in part because of potential