产品名称
Mucinase SmE, mass spec suitable, from Serratia marcescens, recombinant, liquid
recombinant
expressed in E. coli
Quality Level
form
liquid
shelf life
2 yr (Retest)
mol wt
98 kDa
suitability
suitable for mass spectrometry
shipped in
dry ice
storage temp.
-10 to -25°C
General description
Mucinase SmE is a mucin-selective protease that has been engineered to have high activity and and tolerance for site ocupancy and glycoform complexity. Mucinase SmE provides a complementary cleavage profile to Mucinase StcE (Cat No SAE0202). Contrary to StcE digestion that requires an additional tryptic digest prior to MS peptide analysis, when using Mucinase SmE analysis can be done with SmE alone. It is recommended to perform both options to obtain maximal coverage.
Application
Mucinase SmE is useful for glycoproteomics experimentation. It is designed for digestion of densely glycosylated mucin proteins for mass spec based analyses.
Features and Benefits
- Mucin-selective Protease
- Cleaves N-terminally to O-link glycosylated Ser/Thr in mucin domains
- Complimentary cleavage profile to Mucinase StcE
- Accommodates complex, dense and sialylated glycoforms
- Recombinant with His-Tag®
Preparation Note
Thaw the Mucinase SmE by brief incubation at 37°C.
The protein solution is stable for 2 weeks at 2-8 °C.
For longer storage, aliquot the protein solution and store at -20 °C.
The enzyme solution should be protected from direct light.
The protein solution is stable for 2 weeks at 2-8 °C.
For longer storage, aliquot the protein solution and store at -20 °C.
The enzyme solution should be protected from direct light.
Legal Information
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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商品
Pretreatment with Mucinase StcE increases glycopeptide identification from mucin samples, enhancing sample preparation efficiency for glycopeptide analysis.