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T0440

Sigma-Aldrich

ELISA 用 3,3′,5,5′-四甲基联苯胺 (TMB) 液体底物系统

peroxidase substrate

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别名:
TMB
NACRES:
NA.83

质量水平

300

形式

liquid

储存温度

2-8°C

相关类别

一般描述

TMB底物是辣根过氧化物酶-HRP的显色底物。

应用

TMB底物可用于利用辣根过氧化物酶结合物进行的ELISA程序。TMB底物会形成可溶的蓝色反应产物，可通过370或655 nm处的吸光度测定。该试剂可与样品中的亚硝酸盐反应，形成紫色的偶氮产物，可在450 nm处测量其吸光度。该反应可利用酸终止，形成黄色反应产物，并可通过450 nm处的光强精确测定。

我们的TMB底物使用方法：
  • 我们的TMB底物以即用型过氧化物酶底物的形式提供，其中的TMB溶于弱酸性缓冲液中。与过氧化物酶反应之前，底物溶液应呈无色至浅蓝绿色。与过氧化物酶反应时，底物系统形成蓝色反应产物。
  • 加入酸终止反应后，测量TMB底物在450 nm处的光强。
  • 我们的TMB底物推荐用于ELISA（微孔）程序，且已应用在各种测定方案中，如作为辣根过氧化物酶抗体偶联物的底物，用于检测亨德拉病毒糖蛋白、十四糖、sIgA和嗜中性白细胞胞外阱(NETs)。
  • 不建议采用3,3′,5,5′-四甲基联苯胺(TMB)液体底物系统用于膜应用。由于TMB底物会产生可溶性反应产物，因此不建议用于组织化学或印迹分析。
注意：TMB底物为光敏材料，应避免接触直射阳光或紫外线。

推荐

产品编号
说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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在网站页面上，产品编号会附带包装尺寸/数量一起显示（例如：T1503-25G）。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

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批号可以在产品标签上"批“ （Lot或Batch）字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。

  • 如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。

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未找到您寻找的产品？

部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。

索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Do I need to add hydrogen peroxide to Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?

    No hydrogen peroxide should be added to product T0440. The product, which contains  a peroxidase substrate,  already contains hydrogen peroxide.  This solution is ready to use for ELISA assays.

  4. What is the concentration of the TMB and what is the solvent in Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?

    The concentration of TMB in this formulation and the identity of the solvent are considered proprietary information. The product is a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer.

  5. Do I need to dilute Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?

    No, the product should not be diluted.. The product is ready-to-use for ELISA applications. If the intensity of the reaction needs to be reduced, it is recommended that the antibodies or  conjugates be diluted. Usage information  is described on our data sheet.

  6. What is the difference between products T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA,  and T8665, 3,3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System?

    Both products are comparable and either one can be used in ELISA procedures. The formulations which are proprietary may have some slight differences.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Denise Traxler et al.
Clinica chimica acta; international journal of clinical chemistry, 473, 127-132 (2017-08-29)
Heat shock proteins (HSPs) represent intracellular mechanisms of stress response. Clinical implications of their (systemic) expression in patients with chronic heart failure (HF) remain inconclusive. In outpatients with chronic stable HF plasma HSP27 levels were measured using ELISA. Patients were
Dorin Harpaz et al.
Diagnostics (Basel, Switzerland), 10(1) (2020-01-16)
Abstract: Paper-based colorimetric biosensors combine the use of paper with colorimetric signal detection. However, they usually demonstrate lower sensitivities because a signal amplification procedure has not been used. Stopping the reaction of colorimetric signal generation is often used in lab-based
Young Children Display Diurnal Patterns of Salivary IgA and Alpha-Amylase Expression Which Are Independent of Food Intake and Demographic Factors
Lim PW, et al.
BioMed Research International, 2019 (2019)
Spontaneous and tetracosactide-induced anti-ACTH antibodies in man
Gan EH, et al.
Clinical Endocrinology, 84(4), 489-495 (2016)
Médea Padra et al.
Virulence, 9(1), 898-918 (2018-04-12)
Helicobacter suis colonizes the stomach of most pigs and is the most prevalent non-Helicobacter pylori Helicobacter species found in the human stomach. In the human host, H. suis contributes to the development of chronic gastritis, peptic ulcer disease and MALT
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