Merck
CN

T1147

Sigma-Aldrich

脱铁转铁蛋白 人

powder, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)

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别名:
人转铁蛋白, 铁传递蛋白
CAS号:
EC 号:
MDL编号:
NACRES:
NA.75

生物来源

human plasma

产品线

BioReagent

检测方案

≥98% (agarose gel electrophoresis)

形式

powder

分子量

76-81 kDa

浓度

~25 mM

技术

cell culture | mammalian: suitable

杂质

HIV and HBsAg, source material tested negative
≤0.005% iron

溶解性

H2O: 50 mg/mL

UniProt登记号

运输

wet ice

储存温度

−20°C

基因信息

human ... TF(7018)

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应用

载脂蛋白转铁蛋白可以补充铁或用于结合培养基中存在的游离铁,还可用于将铁运输到哺乳动物细胞中。

生化/生理作用

转铁蛋白是一种存在于血清中的主要铁结合和细胞传递分子。 无血清细胞培养系统需要一种铁的传递形式。 转铁蛋白是铁的优选传递形式,因为细胞可通过细胞表面上的转铁蛋白受体以生理学上适当的方式对转铁蛋白结合的铁进行处理。 人载脂蛋白-转铁蛋白是一种高亲和力的转铁蛋白,可用于多种细胞类型。 载脂蛋白-转铁蛋白可以在使用前装载铁或直接添加到含铁介质中。

其他说明

非血红素铁转运蛋白。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

法规信息

常规特殊物品

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

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  5. What is the carbohydrate content of human transferrin?

    We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.

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Giang D Nguyen et al.
PloS one, 9(5), e96858-e96858 (2014-05-08)
H1 linker histone proteins are essential for the structural and functional integrity of chromatin and for the fidelity of additional epigenetic modifications. Deletion of H1c, H1d and H1e in mice leads to embryonic lethality by mid-gestation with a broad spectrum
Giang D Nguyen et al.
PloS one, 8(8), e72698-e72698 (2013-08-24)
Huntington's disease (HD) is a neurodegenerative disease caused by abnormal polyglutamine expansion in the huntingtin protein (Htt). Although both Htt and the HD pathogenic mutation (mHtt) are implicated in early developmental events, their individual involvement has not been adequately explored.
Ayumu Suzuki et al.
Nature communications, 7, 11056-11056 (2016-03-31)
Meiosis is a unique process that allows the generation of reproductive cells. It remains largely unknown how meiosis is initiated in germ cells and why non-germline cells do not undergo meiosis. We previously demonstrated that knockdown of Max expression, a
Kamil Strzelak et al.
Analytica chimica acta, 995, 43-51 (2017-11-12)
The Multicommutated Flow Analysis (MCFA) system for the estimation of clinical iron parameters: Serum Iron (SI), Unsaturated Iron Binding Capacity (UIBC) and Total Iron Binding Capacity (TIBC) has been proposed. The developed MCFA system based on simple photometric detection of
Kathleen N O'Guin et al.
ASN neuro, 6(1), e00135-e00135 (2014-01-31)
The molecular requirements for human myelination are incompletely defined, and further study is needed to fully understand the cellular mechanisms involved during development and in demyelinating diseases. We have established a human co-culture model to study myelination. Our earlier observations

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How transferrin and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of transferrin.

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