grade
Molecular Biology
Quality Level
description
Liquid Medium
sterility
sterile; 0.2 μm filtered
form
liquid
technique(s)
microbiological culture: suitable
application(s)
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Terrific Broth是一个被高度引用的用于大肠杆菌培养的微生物生长培养基。 这种营养丰富的微生物肉汤含有甘油、肽、氨基酸、水溶性维生素和碳水化合物。
Application
Terrific肉汤用于非选择性培养大肠杆菌菌株，用于克隆、高产量生产DNA质粒和高产量生产重组蛋白。引用 它也适用于添加适当抗生素时的选择性培养。
Features and Benefits
Terrific Broth液可提供：
- 过滤器灭菌，即用型
- 方便包装规格
- 标准配方
Other Notes
23.6g/L酵母提取物
11.8g/L 胰蛋白胨
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L甘油
11.8g/L 胰蛋白胨
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L甘油
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
动物来源培养基
此项目有
实验方案
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
General protocols for growth of competent cells in microbial medium.
Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.