T5694
TRAIL human
recombinant, expressed in NSO cells, >97% (SDS-PAGE), lyophilized powder
别名:
Apo2 Ligand, Apo2L, TL2, TNF-related apoptosis-inducing ligand
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关于此项目
Form:
lyophilized powder
Assay:
>97% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in NSO cells
Mol wt:
24 kDa by SDS-PAGE, predicted mol wt ~21 kDa
biological source
human
Quality Level
recombinant
expressed in NSO cells
description
N-terminal 6X histidine-tagged extracellular domain
assay
>97% (SDS-PAGE)
form
lyophilized powder
mol wt
24 kDa by SDS-PAGE, predicted mol wt ~21 kDa
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... TNFSF10(8743)
Application
TRAIL human was used to induce cytotoxicity in A549, a human lung carcinoma cell line.
Biochem/physiol Actions
TNF-related apoptosis-inducing ligand (TRAIL) is a type II transmembrane protein that belongs to TNF family. It is present in both soluble as well as membrane-bound form and can induce rapid apoptosis of various transformed cell lines but not the normal cells. Additionally, the product can also induces apoptotic cell death in TRAIL-sensitive U343MGcells.
Type II transmembrane protein of the TNF superfamily. Ligand for apoptosis-inducing death receptors DR4 and DR5, decoy receptors DcR1 and DcR2, and osteoprotegerin. Approximately 28% amino acid sequence identity to FAS ligand. Human and mouse TRAIL share 65% sequence identity.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.
Analysis Note
Induces cytotoxicity in mouse L-929 cells in the presence of actinomycin D.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
低风险生物材料
此项目有
Matthew J Coussens et al.
Journal of visualized experiments : JoVE, (58)(58), doi:10-doi:10 (2012-01-05)
RNA interference (RNAi) is an intrinsic cellular mechanism for the regulation of gene expression. Harnessing the innate power of this system enables us to knockdown gene expression levels in loss of gene function studies. There are two main methods for
J Sträter et al.
Annals of the New York Academy of Sciences, 915, 162-170 (2001-02-24)
The tumor necrosis factor receptor (TNFR) family is a still-growing group of homologous transmembrane proteins, some of which bear an intracellular "death domain" and are able to directly mediate apoptosis. Apoptosis is induced upon trimerization of the receptors by their
W S Simonet et al.
Cell, 89(2), 309-319 (1997-04-18)
A novel secreted glycoprotein that regulates bone resorption has been identified. The protein, termed Osteoprotegerin (OPG), is a novel member of the TNF receptor superfamily. In vivo, hepatic expression of OPG in transgenic mice results in a profound yet nonlethal
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T5694-10UG | 04061833236987 |