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安全信息

T6674

Sigma-Aldrich

肿瘤坏死因子-α 人

TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

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别名:
hTNF-α, TNF-α
CAS号:
94948-59-1
MDL编号:
MFCD00148692
NACRES:
NA.77

生物来源

human

质量水平

200

重组

expressed in E. coli

检测方案

≥97% (SDS-PAGE)

形式

powder

效能

0.02-0.3 ng/mL ED50/EC50

质量

endotoxin tested

分子量

~17.4 kDa

包装

pkg of 5x10 μg
pkg of 10 μg

储存条件

avoid repeated freeze/thaw cycles

技术

cell culture | mammalian: suitable

杂质

<1 EU/μg

UniProt登记号

P01375

储存温度

−20°C

基因信息

human ... TNF(7124)

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相关类别

应用

TNF-α包括了刺激人成纤维细胞和其他细胞系生长，激活多形核中性粒细胞与破骨细胞，以及诱导白细胞介素-1、前列腺素E2和胶原酶的产生。
人肿瘤坏死因子-α (TNFα)已用于：
  • 分析细胞因子TNFα-刺激人类神经细胞和神经胶质（HNG）细胞的作用
  • 研究TNFα-介导的脯氨酰4羟化酶α1 (P4Hα1)抑制分子机制
  • 诱导死亡-受体介导的HeLa细胞凋亡。

生化/生理作用

肿瘤坏死因子-α（TNF-α），又称为恶病质素(Cachectin)，是一种分子量为26kDa的膜结合蛋白，可被TNF-α转化酶（TACE）切割并释放可溶性17kDa单体，这些单体在循环中形成同源三聚体。TNF-α参与抗肿瘤活性、免疫调节、炎症、厌食、恶病质、脓毒性休克、病毒复制和造血等过程。TNF-α由多种细胞表达，具有许多诱导剂和抑制剂。TNF-α主要由被免疫应激（如细菌、脂多糖）、病毒、寄生虫、促分裂原和其他细胞因子）激活的巨噬细胞产生。TNF-α对许多转化细胞具有细胞毒性作用（活性与其名称相同），但在正常二倍体细胞中，它能够刺激增殖（成纤维细胞）、分化（骨髓细胞）或活化（中性粒细胞）。 TNF-α还对DNA和RNA病毒具有抗病毒作用，并且可诱导多种其他细胞因子的产生。尽管TNF-α在临床试验中被用作抗肿瘤剂，但Sigma的细胞因子、生长因子和激素产品仅限研究使用。TNF-α和相关分子TNF-β(LT-α)具有紧密的结构同源性，它们具有28％的相同氨基酸序列，并且可激活相同的TNF受体，即TNFR1和TNFR2。 小鼠和人TNF-α具有79％的相同氨基酸序列。与人TNF-α不同，小鼠TNF-α为N-糖基化形式。

外形

从含有3mM Tris-HCl和5%海藻糖的0.2 μm-过滤缓冲液中得到冻干粉。

分析说明

通过小鼠L929细胞溶解，检测了TNF-α的生物活性。

法规信息

常规特殊物品

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T1503
货号
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  • 如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。

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索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the package size of Product T6674, Tumor Necrosis Factor-α human?

    It is a 10μg size-power form.

  6. What are the units/mg of Product T6674, Tumor Necrosis Factor-α human?

    1ug=1.1x10e5 units.

  7. What is the solubility of this Product T6674, Tumor Necrosis Factor-α human?

    This can be dissolved at 0.1 to 1.0 mg/ml in saline or buffer, stabilized with 0.1 to 1%BSA.

  8. What is the EC50 of Product T6674, Tumor Necrosis Factor-α human?

    Bioactivity(cell inhibition, L929 cells) is expressed by an EC50 of 0.02 to 0.05 ng/mL for T6674.  EC50 (equivalent concentration) is the same as ED50 (equivalent dose, cytotoxicity, L929 cells) used for the bioactivity specification for T0157, Tumor Necrosis Factor-alpha, human recombinant expressed in Yeast.       

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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