Merck
CN

T8158

Sigma-Aldrich

转铁蛋白 人

powder, BioReagent, suitable for cell culture

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别名:
转铁蛋白,部分饱和
CAS号:
EC 号:
MDL编号:
NACRES:
NA.75

产品线

BioReagent

检测方案

≥98%

形式

powder

浓度

~25 mM

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested
hepatitis and HIV antibody, none detected

溶解性

H2O: 50 mg/mL

UniProt登记号

运输

ambient

储存温度

−20°C

基因信息

human ... TF(7018)

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一般描述

转铁蛋白是具有分支糖链的糖蛋白,多肽链中具有两个铁离子结合位点。

应用

人转铁蛋白已用于:
  • 与IgG一起孵育人近端肾小管细胞 (HKC)-8个细胞,检验白蛋白特异性
  • 用作人糖原细胞培养的含血清/无人甲状腺细胞培养液的补充剂
  • 用作开发凝胶肼解法的靶糖蛋白

生化/生理作用

转铁蛋白介导铁从血浆向细胞的运输。与铁结合的转铁蛋白具有三种形式:单铁A/B转铁蛋白和二铁转铁蛋白。转铁蛋白可与特定的细胞表面受体结合,通过内吞作用内化。进入细胞后,铁被释放到细胞中,无铁转铁蛋白(也称为脱铁转铁蛋白)则通过胞吐作用释放到细胞外,而不会被降解。目前确知转铁蛋白可将铁以生理学安全有效的形式递送至细胞。

其他说明

部分铁饱和

制备说明

60℃热处理10小时。

分析说明

通过琼脂糖电泳纯化。

相关产品

产品编号
说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

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示例

T1503
货号
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25G
包装规格/数量

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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

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  5. What is the carbohydrate content of human transferrin?

    We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.

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Martijn F Hoes et al.
European journal of heart failure, 20(5), 910-919 (2018-02-28)
Iron deficiency is common in patients with heart failure and associated with a poor cardiac function and higher mortality. How iron deficiency impairs cardiac function on a cellular level in the human setting is unknown. This study aims to determine
Cells: Molecules and Mechanisms, 174-174 (2009)
Detection of N-glycans on small amounts of glycoproteins in tissue samples and sodium dodecyl sulfate-polyacrylamide gels
Yoshimura T, et al.
Analytical Biochemistry, 423(2), 253-260 (2012)
Non-transferrin bound iron: a key role in iron overload and iron toxicity
Brissot P, et al.
Biochim. Biophys. Acta Gen. Subj., 1820(3), 403-410 (2012)
Glucose-6-phosphate dehydrogenase activity in monolayer cultures of thyroid epithelial cells: TSH and inhibition of nitrogen oxide synthase affect the enzyme activity and the oxygen sensitivity of the histochemical assay
Kayser L and Thomsen J
Acta Histochemica, 107(1), 31-41 (2005)

商品

How transferrin and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of transferrin.

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