Merck
CN
所有图片(3)

文件

安全信息

WGA4

Sigma-Aldrich

GenomePlex® 单细胞全基因组扩增试剂盒

Amplify genome of a single cell

登录查看公司和协议定价
所有图片(3)
别名:
Single cell whole genome amplification, Whole genome amplification
EC 号:
254-457-8
NACRES:
NA.55

技术

whole genome amplification: suitable

运输

wet ice

储存温度

−20°C

相关类别

一般描述

GenomePlex® 单细胞全基因组扩增试剂盒采用基于基因组 DNA 随机片段化的专有技术，并将产生的小片段转换为两侧有通用引物结合位点的 PCR 可扩增文库分子。 WGA 采用通用寡核苷酸引物进行文库分子 PCR 扩增。该试剂盒旨在扩增单个细胞的基因组。这种快速直接的方法实现了百万倍的扩增，从单个细胞制备微克级基因组 DNA。传统单细胞全基因组扩增方法得率不足，且存在明显偏差。该试剂盒包含细胞裂解和全基因组扩增所需的全部试剂。可以通过荧光激活细胞分选 (FACS)、激光捕获显微切割 (LCM)、稀释或任何其它适用方法分离单个细胞。

应用

GenomePlex® 单细胞全基因组扩增试剂盒已用于：

  • 扩增分离的 DNA
  • 全基因组扩增（WGA）
  • 扩增显微切割的 DNA
  • 从移性性去势抵抗性前列腺癌细胞中扩增循环肿瘤细胞基因组 DNA
  • 通过单细胞低深度全基因组测序 （SLWGS）方法检测拷贝数变异 （CNV）
  • 凝胶电泳
  • qPCR
  • 比较基因组杂交（CGH）微阵列
  • 短串联重复序列（STR）分析
  • 单核苷酸多态性（SNP）分析

特点和优势

  • 4 小时内高产量和准确的DNA 扩增
  • 扩增任何来源的 DNA，例如癌细胞、上皮细胞、淋巴细胞、成纤维细胞羊膜细胞、聚碳酸酯固定细胞和植物细胞
  • 完整表达整个基因组，等位基因偏差最小
  • WGA DNA 聚合酶适用于下游应用，包括凝胶电泳、qPCR、比较基因组杂交（CGH）微阵列、短串联重复序列（STR）分析和单核苷酸多态性（SNP）分析、TaqMan ®分析和微卫星分析

其他说明

此试剂盒中提供的通用引物序列被认为是专有的。

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护： 5,789,224、5,618,711、6,127,155 和美国境外的权利要求，对应于过期的美国专利号 5,079,352。 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者′内部的研究用途。未通过明示、暗示或禁止反言形式授予购买者任何专利主张下的权利、执行任何专利方法的权利或开展任何形式的商业服务的权利，包括但不限于出于经济利益或其他商业考虑报告购买者′的活动结果。本品仅供研究使用。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • L104310x Single Cell Lysis & Fragmentation Buffer化学品安全说明书
  • P4850Proteinase K from Tritirachium album, buffered aqueous glycerol solution, for molecular biology, ≥800 units/mL化学品安全说明书
  • W4502Water, Nuclease-Free Water, for Molecular Biology化学品安全说明书

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

为方便起见，与您过往购买产品相关的文件已保存在文档库中。

访问文档库

难以找到您所需的产品或批次号码？

在网站页面上，产品编号会附带包装尺寸/数量一起显示（例如：T1503-25G）。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题？欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ （Lot或Batch）字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。

  • 如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。

  • 如果您查询到的批号含有填充代码（例如 STBB0728K9），请输入去除填充代码K9的批号：STBB0728。

未找到您寻找的产品？

部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。

索取COA

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can the amplified Omniplex products resulting from the use of Product WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit, be used for TA cloning?

    Yes.  Please ensure that a final extension of 7-30 minutes at 72°C is included after amplification if performing subsequent TA cloning reactions.

  4. Can Product WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit, be used with purified DNA?

    Yes. The kit is designed to amplify the small amount of DNA that is present in a single cell (3-5 pg). It will also work with small amounts of purified DNA; we recommend less than 100 pg.

  5. What kinds of cells can be used as starting materials with Product WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit?

    The cell lysis method involves treatment with proteinase K and heat. Most cells will be lysed under these conditions.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Nathan R Treff et al.
Molecular human reproduction, 16(8), 583-589 (2010-05-21)
Many studies estimate that chromosomal mosaicism within the cleavage-stage human embryo is high. However, comparison of two unique methods of aneuploidy screening of blastomeres within the same embryo has not been conducted and may indicate whether mosaicism has been overestimated
Nathan R Treff et al.
Fertility and sterility, 94(6), 2017-2021 (2010-03-02)
To develop and validate a whole genome amplification and single nucleotide polymorphism (SNP) microarray protocol for accurate single cell 24 chromosome aneuploidy screening. Prospective, randomized, and blinded study. Academic reproductive medicine center. Multiple euploid and aneuploid cell lines were obtained
Resolving tumor heterogeneity: genes involved in chordoma cell development identified by low-template analysis of morphologically distinct cells
El-Heliebi A, et al.
PLoS ONE, 9(2), e87663-e87663 (2014)
Heike Fiegler et al.
Nucleic acids research, 35(3), e15-e15 (2006-12-21)
Heterogeneity in the genome copy number of tissues is of particular importance in solid tumor biology. Furthermore, many clinical applications such as pre-implantation and non-invasive prenatal diagnosis would benefit from the ability to characterize individual single cells. As the amount
Jochen B Geigl et al.
Nature protocols, 2(12), 3173-3184 (2007-12-15)
A comprehensive genomic analysis of single cells is instrumental for numerous applications in tumor genetics, clinical diagnostics and forensic analyses. Here, we provide a protocol for single-cell isolation and whole genome amplification, which includes the following stages: preparation of single-cell
查看所有相关文献

商品

Histone Modification and Chromatin Remodeling | Epigenetics

Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.

Histone Modification and Chromatin Remodeling | Epigenetics

Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.

实验方案

GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol

GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA

GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol

GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA

Whole Genome Amplification from Serum or Plasma Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

Whole Genome Amplification from Serum or Plasma Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

查看所有结果

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门