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Extract-N-Amp™植物PCR试剂盒

sufficient for 100 extractions, sufficient for 500 amplifications

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别名:
Plant direct PCR kit
NACRES:
NA.55

用途

sufficient for 100 extractions
 sufficient for 500 amplifications
 sufficient for 500 reactions

质量水平

200

特点

dNTPs included
hotstart

技术

PCR: suitable

颜色

colorless

运输

wet ice

储存温度

−20°C

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相关类别

一般描述

Extract-N-Amp™植物PCR试剂盒用于直接PCR（聚合酶链反应）。该试剂盒提供了从植物叶片快速提取基因组DNA以及通过PCR扩增靶标所需的所有试剂。它是一种创新的提取解决方案，无需常规的植物组织冷冻处理。
The PCR reaction mix facilitates amplification directly from the extract by using an antibody-based hot-start DNA polymerase for specific amplification.

应用

Extract-N-Amp™植物PCR试剂盒适用于基因分型。

它已被用于从新鲜蘑菇中提取DNA。
Extract-N-Amp™ Plant PCR Kit has been used to extract plant genomic DNA and in polymerase chain reaction (PCR).

特点和优势

  • PCR用植物基因组DNA可以在15分钟内提取。
  • 无需冷冻、机械破碎、有机溶剂萃取、柱纯化或沉淀
  • 经过特别配制的PCR ReadyMix 和提取的DNA一起使用
  • 热启动抗体确保基因组DNA的高度特异性PCR扩增
  • 适用于高通量植物基因分析
  • 无需长时间的酶消化，节省时间和精力
  • 提取的DNA在4 °C 下至少6个月保持稳定。

组分

Extract-N-Amp™植物PCR试剂盒包括稀释溶液、提取溶液和Extract-N-Amp PCR 反应混合物（2X）（缓冲液、盐、dNTP、Taq聚合酶和JumpStart Taq抗体）。
Extraction Solution- E7526-12 mL

Dilution Solution- D5688-12mL

Extract-N-Amp PCR ReadyMix. This 2X PCR reaction mix contains buffer, salts, dNTPs, Taq polymerase, and JumpStart Taq antibody.- E3004- 5 x 1.2 ml

Collection tubes

其他说明

Extract-N-Amp植物PCR试剂盒仅供实验室使用。不可用于药物、家庭或其他用途。
The Extract-N-Amp™ Plant PCR Kits are for laboratory use only. Not for drug, household, or other uses.

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

法律信息

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Sophie Lev et al.
Molecular plant-microbe interactions : MPMI, 22(9), 1093-1103 (2009-08-07)
Phosphorylated mitogen-activated protein kinases (MAPK) transmit signals by activation of their targets. The extent of signal transduction could depend on MAPK phosphorylation level, concentration, and subcellular localization. The pathogenicity MAPK Chk1 of the fungal corn pathogen Cochliobolus heterostrophus is required
Noa Eliahu et al.
Eukaryotic cell, 6(3), 421-429 (2007-01-24)
The maize pathogen Cochliobolus heterostrophus requires two mitogen-activated protein kinases (MAPKs), Chk1 and Mps1, to produce normal pigmentation. Young colonies of mps1 and chk1 deletion mutants have a white and autolytic appearance, which was partially rescued by a hyperosmotic environment.
S A Weller et al.
Journal of microbiological methods, 70(2), 379-383 (2007-06-26)
Real-time (TaqMan) PCR assays were developed to detect the strawberry angular leaf spot pathogen Xanthomonas fragariae (Xf) and the strawberry bacterial blight pathogen Xanthomonas arboricola pv. fragariae (Xaf). The Xf PCR (Xf gyrB) was designed within regions of the gyraseB
Rena Shimizu et al.
Plant physiology, 149(2), 841-850 (2008-12-17)
The WUSCHEL-related homeobox (WOX) gene PRESSED FLOWER1 (PRS1) performs a conserved function during lateral organ development in Arabidopsis (Arabidopsis thaliana). Expressed in the periphery of the shoot meristem, PRS1 recruits founder cells that form lateral domains of vegetative and floral
Len N Gillman et al.
Journal of evolutionary biology, 23(6), 1327-1330 (2010-05-12)
A faster rate of nuclear DNA evolution has recently been found for plants occupying warmer low latitudes relative to those in cooler high latitudes. That earlier study by our research group compared substitution rates within the variable internal transcribed spacer
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商品

Extract-N-Amp™ Plant Tissue PCR Kits

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.

Extract-N-Amp™ Plant Tissue PCR Kits

The Extract-N-Amp™ kits are designed to rapidly extract and amplify genomic DNA. The plant tissue version of these kits has been optimized to amplify without concern over plant inhibitors. This technical document will discuss the versions of this kit that are available and help you find the best kit suited for your needs.

基因组DNA样品纯化和质量评估

简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

实验方案

Extract-N-Amp™ Plant PCR Kits Protocol

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

Extract-N-Amp™ Plant PCR Kits Protocol

The Extract-N-Amp™ Plant PCR Kits contain all of the reagents required to rapidly extract and amplify genomic DNA from plant leaves.

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